breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Read File Information

read filereadsbasespassed filtersaveragelongestmapped
errorsJBEISALT_PLXW_3Strain_A_3_F_82_I1_S243_ME_L001_R1_001.good.fq2,575,115362,649,280100.0%140.8 bases141 bases94.0%
errorsJBEISALT_PLXW_3Strain_A_3_F_82_I1_S243_ME_L001_R2_001.good.fq2,575,115362,649,280100.0%140.8 bases141 bases97.3%
total5,150,230725,298,560100.0%140.8 bases141 bases95.7%

Reference Sequence Information

seq idlengthfit meanfit dispersion% mapped readsdescription
coveragedistributionNC_0009134,641,652130.19.4100.0%Escherichia coli str. K-12 substr. MG1655, complete genome.
total4,641,652100.0%

fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.

New Junction Evidence

Junction Candidates Tested

optionlimitactual
Number of alignment pairs examined for constructing junction candidates≤ 10000089122
Coverage evenness (position-hash) score of junction candidates≥ 2≥ 2
Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold100 ≤ n ≤ 50001046
Total length of all junction candidates (factor times the reference genome length)≤ 0.10.066

Junction Skew Score Calculation

reference sequencepr(no read start)
NC_0009130.80642

pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.

Final Junction Predictions

optionvalue
Coverage evenness (position-hash) score of predicted junctions must be≥ 3
Minimum probablilty assigned that no mapped read will start at a given position and strand for junction prediction0.1
Junction allow suboptimal matchesFALSE
Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be≤ 3
Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction≥ 1

Read Alignment Evidence

optionvalue
ModeConsensus/Mixed Base
Ploidy1 (haploid)
Consensus mutation E-value cutoff10
Consensus frequency cutoff0.75
Consensus minimum variant coverage each strandOFF
Consensus minimum total coverage each strandOFF
Consensus minimum variant coverageOFF
Consensus minimum total coverageOFF
Polymorphism E-value cutoff10
Polymorphism frequency cutoff0.2
Polymorphism minimum variant coverage each strandOFF
Polymorphism minimum total coverage each strandOFF
Polymorphism minimum variant coverageOFF
Polymorphism minimum total coverageOFF
Polymorphism bias cutoffOFF
Predict indel polymorphismsYES
Skip indel polymorphisms in homopolymers runs ofOFF
Skip base substitutions when they create a homopolymer flanked on each side byOFF

Software Versions

programversion
bowtie22.2.6
R3.4.4

Execution Times

stepstartendelapsed
Read and reference sequence file input20:24:52 03 Feb 202220:26:16 03 Feb 20221 minute 24 seconds
Read alignment to reference genome20:26:17 03 Feb 202220:37:25 03 Feb 202211 minutes 8 seconds
Preprocessing alignments for candidate junction identification20:37:25 03 Feb 202220:38:36 03 Feb 20221 minute 11 seconds
Preliminary analysis of coverage distribution20:38:36 03 Feb 202220:40:44 03 Feb 20222 minutes 8 seconds
Identifying junction candidates20:40:44 03 Feb 202220:41:11 03 Feb 202227 seconds
Re-alignment to junction candidates20:41:11 03 Feb 202220:45:09 03 Feb 20223 minutes 58 seconds
Resolving best read alignments20:45:09 03 Feb 202220:46:53 03 Feb 20221 minute 44 seconds
Creating BAM files20:46:53 03 Feb 202220:48:45 03 Feb 20221 minute 52 seconds
Tabulating error counts20:48:45 03 Feb 202220:49:45 03 Feb 20221 minute 0 seconds
Re-calibrating base error rates20:49:45 03 Feb 202220:49:46 03 Feb 20221 second
Examining read alignment evidence20:49:46 03 Feb 202221:01:18 03 Feb 202211 minutes 32 seconds
Polymorphism statistics21:01:18 03 Feb 202221:01:19 03 Feb 20221 second
Output21:01:19 03 Feb 202221:01:35 03 Feb 202216 seconds
Total 36 minutes 42 seconds