breseq version 0.35.4 revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Marginal read alignment evidence (highest frequency 20 of 51 shown, sorted by frequency from high to low) | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NZ_CP034943 | 3,285,647 | 0 | C | A | 42.9% | 211.7 / inf | 333 | V252L (GTA→TTA) | EO946_RS16995 | IS3 family transposase |
* | NZ_CP034943 | 3,285,713 | 0 | G | A | 37.7% | 196.8 / 198.2 | 168 | L230L (CTA→TTA) | EO946_RS16995 | IS3 family transposase |
* | NZ_CP034943 | 3,285,580 | 0 | A | G | 37.0% | 303.1 / inf | 284 | I274T (ATA→ACA) | EO946_RS16995 | IS3 family transposase |
* | NZ_CP034943 | 3,285,575 | 0 | T | G | 34.6% | 360.5 / 279.4 | 286 | N276H (AAT→CAT) | EO946_RS16995 | IS3 family transposase |
* | NZ_CP034943 | 3,285,567 | 0 | C | T | 33.6% | 370.6 / 302.4 | 292 | L278L (CTG→CTA) | EO946_RS16995 | IS3 family transposase |
* | NZ_CP034943 | 1,730,096 | 0 | G | C | 32.2% | 178.6 / 36.6 | 122 | E173Q (GAA→CAA) | rodZ | cell shape determination protein RodZ |
* | NZ_CP034943 | 2,991,236 | 0 | C | G | 31.7% | 288.3 / 45.8 | 169 | intergenic (+37/‑170) | gbsR/bslA | transcriptional regulator GbsR/biofilm‑surface layer protein BslA |
* | NZ_CP034943 | 2,991,252 | 0 | C | A | 29.9% | 283.1 / 31.5 | 167 | intergenic (+53/‑154) | gbsR/bslA | transcriptional regulator GbsR/biofilm‑surface layer protein BslA |
* | NZ_CP034943 | 634,054 | 0 | C | G | 28.8% | 263.8 / 38.2 | 143 | E573Q (GAA→CAA) | EO946_RS03330 | ABC‑F family ATP‑binding cassette domain‑containing protein |
* | NZ_CP034943 | 2,991,227 | 0 | T | G | 27.8% | 268.7 / 26.4 | 170 | intergenic (+28/‑179) | gbsR/bslA | transcriptional regulator GbsR/biofilm‑surface layer protein BslA |
* | NZ_CP034943 | 177,053 | 0 | C | A | 27.7% | 271.8 / 24.0 | 155 | intergenic (+226/‑46) | EO946_RS01020/EO946_RS01025 | alcohol dehydrogenase/SulP family inorganic anion transporter |
* | NZ_CP034943 | 3,446,970 | 0 | G | T | 27.0% | 336.6 / 59.0 | 205 | Q206K (CAA→AAA) | degU | two‑component system response regulator DegU |
* | NZ_CP034943 | 2,103,765 | 0 | C | A | 26.7% | 177.6 / 17.2 | 107 | V7L (GTA→TTA) | azoJ | FMN‑dependent NADH‑azoreductase AzoJ |
* | NZ_CP034943 | 1,966,258 | 0 | G | C | 26.6% | 199.0 / 21.4 | 116 | intergenic (+1/‑452) | iseA/EO946_RS09770 | DL‑endopeptidase inhibitor IseA/hypothetical protein |
* | NZ_CP034943 | 1,730,075 | 0 | G | C | 26.3% | 240.1 / 19.0 | 126 | E166Q (GAA→CAA) | rodZ | cell shape determination protein RodZ |
* | NZ_CP034943 | 1,111,649 | 0 | C | A | 26.1% | 295.1 / 21.1 | 149 | P582T (CCG→ACG) | addB | helicase‑exonuclease AddAB subunit AddB |
* | NZ_CP034943 | 549,113 | 0 | G | C | 25.4% | 413.9 / 29.9 | 196 | R42T (AGA→ACA) | EO946_RS02865 | PLP‑dependent aminotransferase family protein |
* | NZ_CP034943 | 2,991,245 | 0 | G | T | 25.1% | 284.4 / 44.7 | 171 | intergenic (+46/‑161) | gbsR/bslA | transcriptional regulator GbsR/biofilm‑surface layer protein BslA |
* | NZ_CP034943 | 549,080 | 0 | G | T | 25.1% | 421.8 / 25.9 | 212 | R31L (CGT→CTT) | EO946_RS02865 | PLP‑dependent aminotransferase family protein |
* | NZ_CP034943 | 1,366,109 | 0 | G | T | 24.5% | 307.5 / 14.2 | 170 | D203Y (GAT→TAT) | EO946_RS07135 | PepSY domain‑containing protein |
Marginal new junction evidence (lowest skew 10 of 20 shown) | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | NZ_CP034943 | = 607483 | 213 (0.950) | 8 (0.040) | 6/242 | 8.6 | 3.8% | coding (326/1125 nt) | EO946_RS03185 | collagen‑like protein |
? | NZ_CP034943 | 607727 = | 215 (1.070) | coding (570/1125 nt) | EO946_RS03185 | collagen‑like protein | |||||
* | ? | NZ_CP034943 | 607695 = | 158 (0.710) | 9 (0.040) +AGTAGCCCCT |
6/250 | 8.8 | 4.4% | coding (538/1125 nt) | EO946_RS03185 | collagen‑like protein |
? | NZ_CP034943 | = 607846 | 264 (1.180) | coding (689/1125 nt) | EO946_RS03185 | collagen‑like protein | |||||
* | ? | NZ_CP034943 | 607713 = | 224 (1.000) | 11 (0.050) | 6/260 | 9.1 | 4.6% | coding (556/1125 nt) | EO946_RS03185 | collagen‑like protein |
? | NZ_CP034943 | = 607721 | 238 (1.100) | coding (564/1125 nt) | EO946_RS03185 | collagen‑like protein | |||||
* | ? | NZ_CP034943 | 767776 = | 234 (1.050) | 5 (0.020) | 5/244 | 9.2 | 2.2% | coding (189/645 nt) | EO946_RS03930 | hypothetical protein |
? | NZ_CP034943 | = 767772 | 224 (1.110) | coding (193/645 nt) | EO946_RS03930 | hypothetical protein | |||||
* | ? | NZ_CP034943 | = 165756 | 262 (1.170) | 4 (0.020) | 4/224 | 9.3 | 1.5% | noncoding (65/73 nt) | EO946_RS00965 | tRNA‑Thr |
? | NZ_CP034943 | 166185 = | 300 (1.620) | noncoding (66/76 nt) | EO946_RS00985 | tRNA‑Ala | |||||
* | ? | NZ_CP034943 | = 607225 | 303 (1.350) | 6 (0.030) +AGGGGCTACTGGGGACGT |
4/234 | 9.5 | 2.8% | coding (68/1125 nt) | EO946_RS03185 | collagen‑like protein |
? | NZ_CP034943 | 607703 = | 180 (0.800) | coding (546/1125 nt) | EO946_RS03185 | collagen‑like protein | |||||
* | ? | NZ_CP034943 | = 607355 | 241 (1.080) | 8 (0.040) +GCGACAGGAGCTACC |
4/240 | 9.7 | 4.4% | coding (198/1125 nt) | EO946_RS03185 | collagen‑like protein |
? | NZ_CP034943 | 607587 = | 153 (0.680) | coding (430/1125 nt) | EO946_RS03185 | collagen‑like protein | |||||
* | ? | NZ_CP034943 | = 607745 | 254 (1.130) | 4 (0.020) +21 bp |
3/228 | 10.0 | 1.7% | coding (588/1125 nt) | EO946_RS03185 | collagen‑like protein |
? | NZ_CP034943 | 607839 = | 293 (1.310) | coding (682/1125 nt) | EO946_RS03185 | collagen‑like protein | |||||
* | ? | NZ_CP034943 | = 607673 | 149 (0.670) | 4 (0.020) +21 bp |
3/228 | 10.0 | 2.1% | coding (516/1125 nt) | EO946_RS03185 | collagen‑like protein |
? | NZ_CP034943 | 607839 = | 293 (1.310) | coding (682/1125 nt) | EO946_RS03185 | collagen‑like protein | |||||
* | ? | NZ_CP034943 | = 607561 | 199 (0.890) | 5 (0.030) +ACCAACCGGAGCGACG |
3/238 | 10.3 | 2.3% | coding (404/1125 nt) | EO946_RS03185 | collagen‑like protein |
? | NZ_CP034943 | 607839 = | 292 (1.300) | coding (682/1125 nt) | EO946_RS03185 | collagen‑like protein |