mutation predictions | marginal predictions | summary statistics | genome diff | command line log
|
breseq version 0.35.4 revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
| Marginal read alignment evidence (highest frequency 20 of 44 shown, sorted by frequency from high to low) | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP034943 | 3,285,647 | 0 | C | A | 46.4% | 140.2 / inf | 290 | V252L (GTA→TTA) | EO946_RS16995 | IS3 family transposase |
| * | NZ_CP034943 | 634,054 | 0 | C | G | 39.5% | 211.4 / 77.5 | 159 | E573Q (GAA→CAA) ‡ | EO946_RS03330 | ABC‑F family ATP‑binding cassette domain‑containing protein |
| * | NZ_CP034943 | 3,285,713 | 0 | G | A | 38.8% | 164.1 / 160.1 | 136 | L230L (CTA→TTA) | EO946_RS16995 | IS3 family transposase |
| * | NZ_CP034943 | 3,285,580 | 0 | A | G | 38.8% | 269.6 / inf | 295 | I274T (ATA→ACA) | EO946_RS16995 | IS3 family transposase |
| * | NZ_CP034943 | 3,285,575 | 0 | T | G | 35.6% | 344.6 / 312.8 | 306 | N276H (AAT→CAT) | EO946_RS16995 | IS3 family transposase |
| * | NZ_CP034943 | 3,285,567 | 0 | C | T | 35.0% | 354.3 / inf | 307 | L278L (CTG→CTA) | EO946_RS16995 | IS3 family transposase |
| * | NZ_CP034943 | 2,991,236 | 0 | C | G | 32.9% | 277.7 / 37.9 | 155 | intergenic (+37/‑170) | gbsR/bslA | transcriptional regulator GbsR/biofilm‑surface layer protein BslA |
| * | NZ_CP034943 | 2,991,227 | 0 | T | G | 30.7% | 242.6 / 42.9 | 163 | intergenic (+28/‑179) | gbsR/bslA | transcriptional regulator GbsR/biofilm‑surface layer protein BslA |
| * | NZ_CP034943 | 634,058 | 0 | C | G | 29.7% | 269.2 / 38.2 | 160 | K571N (AAG→AAC) | EO946_RS03330 | ABC‑F family ATP‑binding cassette domain‑containing protein |
| * | NZ_CP034943 | 1,730,096 | 0 | G | C | 29.5% | 148.7 / 33.0 | 107 | E173Q (GAA→CAA) | rodZ | cell shape determination protein RodZ |
| * | NZ_CP034943 | 634,052 | 0 | T | G | 28.3% | 257.5 / 34.6 | 159 | E573D (GAA→GAC) ‡ | EO946_RS03330 | ABC‑F family ATP‑binding cassette domain‑containing protein |
| * | NZ_CP034943 | 634,064 | 0 | C | G | 28.2% | 386.7 / 36.3 | 188 | W569C (TGG→TGC) | EO946_RS03330 | ABC‑F family ATP‑binding cassette domain‑containing protein |
| * | NZ_CP034943 | 2,991,262 | 0 | G | T | 27.5% | 236.0 / 29.2 | 133 | intergenic (+63/‑144) | gbsR/bslA | transcriptional regulator GbsR/biofilm‑surface layer protein BslA |
| * | NZ_CP034943 | 2,408,727 | 0 | C | A | 26.8% | 227.7 / 23.1 | 138 | A327S (GCG→TCG) | spoIIIAE | stage III sporulation protein AE |
| * | NZ_CP034943 | 2,991,252 | 0 | C | A | 26.8% | 262.2 / 26.3 | 144 | intergenic (+53/‑154) | gbsR/bslA | transcriptional regulator GbsR/biofilm‑surface layer protein BslA |
| * | NZ_CP034943 | 1,730,075 | 0 | G | C | 25.5% | 197.1 / 19.3 | 112 | E166Q (GAA→CAA) | rodZ | cell shape determination protein RodZ |
| * | NZ_CP034943 | 3,394,367 | 0 | C | G | 25.5% | 457.1 / 28.3 | 226 | G178A (GGA→GCA) | ppaX | pyrophosphatase PpaX |
| * | NZ_CP034943 | 1,730,093 | 0 | G | C | 25.2% | 186.7 / 12.2 | 107 | E172Q (GAA→CAA) | rodZ | cell shape determination protein RodZ |
| * | NZ_CP034943 | 1,730,071 | 0 | G | C | 25.2% | 202.2 / 17.1 | 113 | T164T (ACG→ACC) | rodZ | cell shape determination protein RodZ |
| * | NZ_CP034943 | 1,925,430 | 0 | T | G | 24.1% | 244.1 / 20.7 | 116 | N1876H (AAC→CAC) | EO946_RS09730 | non‑ribosomal peptide synthetase |
| Marginal new junction evidence (lowest skew 10 of 14 shown) | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | NZ_CP034943 | 607443 = | 216 (0.980) | 28 (0.150) +AGTAGCCCCTGTGGCTCCC |
15/230 | 4.7 | 11.4% | coding (286/1125 nt) | EO946_RS03185 | collagen‑like protein |
| ? | NZ_CP034943 | = 607837 | 292 (1.330) | coding (680/1125 nt) | EO946_RS03185 | collagen‑like protein | |||||
| * | ? | NZ_CP034943 | 607389 = | 236 (1.070) | 14 (0.070) +AGTAGCCCCTGTGGCTCCC |
6/230 | 7.7 | 5.8% | coding (232/1125 nt) | EO946_RS03185 | collagen‑like protein |
| ? | NZ_CP034943 | = 607837 | 292 (1.330) | coding (680/1125 nt) | EO946_RS03185 | collagen‑like protein | |||||
| * | ? | NZ_CP034943 | 3592725 = | 204 (0.930) | 4 (0.020) | 4/248 | 9.2 | 2.2% | coding (424/702 nt) | urtE | urea ABC transporter ATP‑binding subunit UrtE |
| ? | NZ_CP034943 | = 3592721 | 172 (0.840) | coding (428/702 nt) | urtE | urea ABC transporter ATP‑binding subunit UrtE | |||||
| * | ? | NZ_CP034943 | = 607682 | 166 (0.770) | 19 (0.090) +ACGGGAGCC |
4/246 | 9.2 | 10.9% | coding (525/1125 nt) | EO946_RS03185 | collagen‑like protein |
| ? | NZ_CP034943 | 607683 = | 166 (0.750) | coding (526/1125 nt) | EO946_RS03185 | collagen‑like protein | |||||
| * | ? | NZ_CP034943 | 607566 = | 187 (0.850) | 25 (0.120) +CGCTCCGGT |
4/250 | 9.2 | 12.4% | coding (409/1125 nt) | EO946_RS03185 | collagen‑like protein |
| ? | NZ_CP034943 | = 607637 | 193 (0.880) | coding (480/1125 nt) | EO946_RS03185 | collagen‑like protein | |||||
| * | ? | NZ_CP034943 | 767776 = | 241 (1.100) | 3 (0.020) | 3/242 | 9.7 | 1.3% | coding (189/645 nt) | EO946_RS03930 | hypothetical protein |
| ? | NZ_CP034943 | = 767772 | 228 (1.150) | coding (193/645 nt) | EO946_RS03930 | hypothetical protein | |||||
| * | ? | NZ_CP034943 | 1437935 = | 142 (0.650) | 3 (0.010) | 3/248 | 9.8 | 2.2% | coding (738/1821 nt) | kinA | sporulation kinase KinA |
| ? | NZ_CP034943 | = 1437931 | 132 (0.650) | coding (734/1821 nt) | kinA | sporulation kinase KinA | |||||
| * | ? | NZ_CP034943 | 607695 = | 178 (0.810) | 5 (0.020) +AGTAGCCCCT |
3/248 | 9.8 | 2.4% | coding (538/1125 nt) | EO946_RS03185 | collagen‑like protein |
| ? | NZ_CP034943 | = 607846 | 271 (1.230) | coding (689/1125 nt) | EO946_RS03185 | collagen‑like protein | |||||
| * | ? | NZ_CP034943 | 607647 = | 182 (0.830) | 15 (0.070) +GGCTCCCGT |
3/246 | 9.8 | 8.5% | coding (490/1125 nt) | EO946_RS03185 | collagen‑like protein |
| ? | NZ_CP034943 | = 607682 | 167 (0.770) | coding (525/1125 nt) | EO946_RS03185 | collagen‑like protein | |||||
| * | ? | NZ_CP034943 | 607641 = | 187 (0.850) | 3 (0.010) | 3/258 | 10.0 | 1.7% | coding (484/1125 nt) | EO946_RS03185 | collagen‑like protein |
| ? | NZ_CP034943 | = 607685 | 162 (0.760) | coding (528/1125 nt) | EO946_RS03185 | collagen‑like protein | |||||