mutation predictions | marginal predictions | summary statistics | genome diff | command line log
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breseq version 0.35.4 revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
| Marginal read alignment evidence (highest frequency 20 of 39 shown, sorted by frequency from high to low) | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP034943 | 2,991,236 | 0 | C | G | 40.6% | 151.0 / 45.2 | 120 | intergenic (+37/‑170) | gbsR/bslA | transcriptional regulator GbsR/biofilm‑surface layer protein BslA |
| * | NZ_CP034943 | 3,285,580 | 0 | A | G | 37.0% | 336.1 / inf | 300 | I274T (ATA→ACA) | EO946_RS16995 | IS3 family transposase |
| * | NZ_CP034943 | 3,285,647 | 0 | C | A | 36.5% | 303.0 / 302.6 | 290 | V252L (GTA→TTA) | EO946_RS16995 | IS3 family transposase |
| * | NZ_CP034943 | 2,991,227 | 0 | T | G | 33.6% | 159.2 / 40.4 | 126 | intergenic (+28/‑179) | gbsR/bslA | transcriptional regulator GbsR/biofilm‑surface layer protein BslA |
| * | NZ_CP034943 | 3,285,575 | 0 | T | G | 32.7% | 427.5 / 268.5 | 303 | N276H (AAT→CAT) | EO946_RS16995 | IS3 family transposase |
| * | NZ_CP034943 | 3,285,567 | 0 | C | T | 32.5% | 428.7 / 308.8 | 305 | L278L (CTG→CTA) | EO946_RS16995 | IS3 family transposase |
| * | NZ_CP034943 | 595,203 | 0 | C | A | 29.8% | 216.3 / 26.8 | 124 | T30K (ACG→AAG) | EO946_RS03130 | MMPL family transporter |
| * | NZ_CP034943 | 634,054 | 0 | C | G | 29.4% | 254.5 / 37.0 | 136 | E573Q (GAA→CAA) | EO946_RS03330 | ABC‑F family ATP‑binding cassette domain‑containing protein |
| * | NZ_CP034943 | 3,446,970 | 0 | G | T | 27.7% | 251.6 / 39.7 | 156 | Q206K (CAA→AAA) | degU | two‑component system response regulator DegU |
| * | NZ_CP034943 | 3,285,713 | 0 | G | A | 26.4% | 248.8 / 91.5 | 127 | L230L (CTA→TTA) | EO946_RS16995 | IS3 family transposase |
| * | NZ_CP034943 | 2,991,245 | 0 | G | T | 26.1% | 190.4 / 27.8 | 119 | intergenic (+46/‑161) | gbsR/bslA | transcriptional regulator GbsR/biofilm‑surface layer protein BslA |
| * | NZ_CP034943 | 2,830,523 | 0 | C | G | 26.0% | 262.8 / 27.5 | 155 | L89V (CTT→GTT) | brnQ | branched‑chain amino acid transport system II carrier protein |
| * | NZ_CP034943 | 3,539,592 | 0 | C | G | 25.6% | 252.3 / 14.7 | 130 | E121Q (GAA→CAA) | EO946_RS18215 | T7SS effector LXG polymorphic toxin |
| * | NZ_CP034943 | 634,058 | 0 | C | G | 25.6% | 279.6 / 17.7 | 135 | K571N (AAG→AAC) | EO946_RS03330 | ABC‑F family ATP‑binding cassette domain‑containing protein |
| * | NZ_CP034943 | 2,408,727 | 0 | C | A | 25.0% | 144.5 / 14.7 | 88 | A327S (GCG→TCG) | spoIIIAE | stage III sporulation protein AE |
| * | NZ_CP034943 | 3,446,980 | 0 | G | T | 24.7% | 300.7 / 41.1 | 162 | S202R (AGC→AGA) | degU | two‑component system response regulator DegU |
| * | NZ_CP034943 | 1,293,348 | 0 | G | T | 24.7% | 314.3 / 13.3 | 155 | V141L (GTA→TTA) | EO946_RS06740 | sigma‑70 family RNA polymerase sigma factor |
| * | NZ_CP034943 | 2,991,252 | 0 | C | A | 24.5% | 188.8 / 14.5 | 110 | intergenic (+53/‑154) | gbsR/bslA | transcriptional regulator GbsR/biofilm‑surface layer protein BslA |
| * | NZ_CP034943 | 1,730,096 | 0 | G | C | 24.3% | 216.0 / 15.7 | 117 | E173Q (GAA→CAA) | rodZ | cell shape determination protein RodZ |
| * | NZ_CP034943 | 2,991,243 | 0 | A | T | 24.3% | 239.1 / 25.7 | 116 | intergenic (+44/‑163) | gbsR/bslA | transcriptional regulator GbsR/biofilm‑surface layer protein BslA |
| Marginal new junction evidence (lowest skew 10 of 19 shown) | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | NZ_CP034943 | = 607528 | 171 (0.920) | 5 (0.050) +65 bp |
3/140 | 6.5 | 5.4% | coding (371/1125 nt) | EO946_RS03185 | collagen‑like protein |
| ? | NZ_CP034943 | 607558 = | 169 (0.910) | coding (401/1125 nt) | EO946_RS03185 | collagen‑like protein | |||||
| * | ? | NZ_CP034943 | 607689 = | 133 (0.720) | 11 (0.060) +CCCGGTG |
9/256 | 6.8 | 7.9% | coding (532/1125 nt) | EO946_RS03185 | collagen‑like protein |
| ? | NZ_CP034943 | = 607690 | 138 (0.740) | coding (533/1125 nt) | EO946_RS03185 | collagen‑like protein | |||||
| * | ? | NZ_CP034943 | = 607256 | 233 (1.250) | 7 (0.040) | 7/264 | 7.9 | 3.3% | coding (99/1125 nt) | EO946_RS03185 | collagen‑like protein |
| ? | NZ_CP034943 | 607428 = | 177 (0.970) | coding (271/1125 nt) | EO946_RS03185 | collagen‑like protein | |||||
| * | ? | NZ_CP034943 | = 607256 | 233 (1.250) | 6 (0.030) | 6/264 | 8.3 | 2.9% | coding (99/1125 nt) | EO946_RS03185 | collagen‑like protein |
| ? | NZ_CP034943 | 607527 = | 171 (0.940) | coding (370/1125 nt) | EO946_RS03185 | collagen‑like protein | |||||
| * | ? | NZ_CP034943 | 2411159 = | 194 (1.040) | 5 (0.030) | 5/250 | 8.5 | 2.7% | coding (635/924 nt) | spoIIIAA | stage III sporulation protein AA |
| ? | NZ_CP034943 | = 2411155 | 182 (1.060) | coding (639/924 nt) | spoIIIAA | stage III sporulation protein AA | |||||
| * | ? | NZ_CP034943 | 607524 = | 175 (0.940) | 4 (0.020) +GGCTGGTCCGACGTCC |
4/238 | 8.8 | 2.3% | coding (367/1125 nt) | EO946_RS03185 | collagen‑like protein |
| ? | NZ_CP034943 | = 607858 | 219 (1.180) | coding (701/1125 nt) | EO946_RS03185 | collagen‑like protein | |||||
| * | ? | NZ_CP034943 | 607479 = | 190 (1.020) | 4 (0.020) +GGCTGGTCCGACGTCC |
4/238 | 8.8 | 2.2% | coding (322/1125 nt) | EO946_RS03185 | collagen‑like protein |
| ? | NZ_CP034943 | = 607858 | 219 (1.180) | coding (701/1125 nt) | EO946_RS03185 | collagen‑like protein | |||||
| * | ? | NZ_CP034943 | 607425 = | 181 (0.970) | 4 (0.020) +GGCTGGTCCGACGTCC |
4/238 | 8.8 | 2.2% | coding (268/1125 nt) | EO946_RS03185 | collagen‑like protein |
| ? | NZ_CP034943 | = 607858 | 219 (1.180) | coding (701/1125 nt) | EO946_RS03185 | collagen‑like protein | |||||
| * | ? | NZ_CP034943 | = 607355 | 220 (1.180) | 12 (0.070) | 5/260 | 8.8 | 5.2% | coding (198/1125 nt) | EO946_RS03185 | collagen‑like protein |
| ? | NZ_CP034943 | 607752 = | 226 (1.260) | coding (595/1125 nt) | EO946_RS03185 | collagen‑like protein | |||||
| * | ? | NZ_CP034943 | = 607256 | 233 (1.250) | 5 (0.030) | 5/264 | 8.8 | 2.4% | coding (99/1125 nt) | EO946_RS03185 | collagen‑like protein |
| ? | NZ_CP034943 | 607482 = | 177 (0.970) | coding (325/1125 nt) | EO946_RS03185 | collagen‑like protein | |||||