breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 52 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,285,5800AG44.7% 160.1 / inf 314I274T (ATA→ACA) EO946_RS16995IS3 family transposase
*NZ_CP0349433,285,6470CA42.0% 230.9 / inf 352V252L (GTA→TTA) EO946_RS16995IS3 family transposase
*NZ_CP0349433,285,5750TG41.8% 231.9 / inf 316N276H (AAT→CAT) EO946_RS16995IS3 family transposase
*NZ_CP0349433,285,5670CT40.2% 231.4 / inf 311L278L (CTG→CTAEO946_RS16995IS3 family transposase
*NZ_CP034943634,0540CG40.0% 127.8 / 44.1 108E573Q (GAA→CAA) ‡EO946_RS03330ABC‑F family ATP‑binding cassette domain‑containing protein
*NZ_CP0349431,730,0750GC35.1% 175.3 / 35.5 115E166Q (GAA→CAA) rodZcell shape determination protein RodZ
*NZ_CP034943634,0580CG34.4% 158.2 / 26.7 108K571N (AAG→AACEO946_RS03330ABC‑F family ATP‑binding cassette domain‑containing protein
*NZ_CP0349431,366,1090GT31.4% 145.6 / 12.1 108D203Y (GAT→TAT) EO946_RS07135PepSY domain‑containing protein
*NZ_CP0349433,285,7130GA30.7% 229.4 / 125.7 139L230L (CTA→TTA) EO946_RS16995IS3 family transposase
*NZ_CP0349432,991,2270TG30.1% 186.6 / 49.9 143intergenic (+28/‑179)gbsR/bslAtranscriptional regulator GbsR/biofilm‑surface layer protein BslA
*NZ_CP0349432,991,2360CG30.0% 232.9 / 35.3 143intergenic (+37/‑170)gbsR/bslAtranscriptional regulator GbsR/biofilm‑surface layer protein BslA
*NZ_CP0349431,730,0660GC29.5% 171.1 / 15.8 112E163Q (GAA→CAA) rodZcell shape determination protein RodZ
*NZ_CP0349431,730,0930GC28.4% 170.5 / 18.6 105E172Q (GAA→CAA) rodZcell shape determination protein RodZ
*NZ_CP0349431,366,1150GC28.0% 179.5 / 18.9 110E205Q (GAA→CAA) EO946_RS07135PepSY domain‑containing protein
*NZ_CP034943634,0640CG26.6% 230.8 / 24.8 129W569C (TGG→TGCEO946_RS03330ABC‑F family ATP‑binding cassette domain‑containing protein
*NZ_CP0349431,730,0710GC26.1% 201.6 / 17.3 115T164T (ACG→ACCrodZcell shape determination protein RodZ
*NZ_CP034943634,0520TG25.9% 165.9 / 14.3 108E573D (GAA→GAC) ‡EO946_RS03330ABC‑F family ATP‑binding cassette domain‑containing protein
*NZ_CP0349432,991,2520CA25.9% 256.8 / 23.7 136intergenic (+53/‑154)gbsR/bslAtranscriptional regulator GbsR/biofilm‑surface layer protein BslA
*NZ_CP0349431,730,0840GC25.4% 208.7 / 16.4 114D169H (GAT→CAT) rodZcell shape determination protein RodZ
*NZ_CP0349431,730,0960GC25.2% 168.3 / 18.4 105E173Q (GAA→CAA) rodZcell shape determination protein RodZ

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP034943 607695 =146 (0.780)29 (0.170)
+AGTAGCCCCT
21/250 3.6 14.8% coding (538/1125 nt) EO946_RS03185 collagen‑like protein
?NZ_CP034943 = 607846 215 (1.150)coding (689/1125 nt) EO946_RS03185 collagen‑like protein
* ? NZ_CP034943 = 607210261 (1.390)16 (0.100)
+AGCGACAGGAGCTACC
7/238 7.3 8.2% coding (53/1125 nt) EO946_RS03185 collagen‑like protein
?NZ_CP034943 607587 = 146 (0.780)coding (430/1125 nt) EO946_RS03185 collagen‑like protein
* ? NZ_CP034943 607659 =140 (0.750)9 (0.050)
+AGTAGCCCCT
6/250 8.0 5.2% coding (502/1125 nt) EO946_RS03185 collagen‑like protein
?NZ_CP034943 = 607846 216 (1.150)coding (689/1125 nt) EO946_RS03185 collagen‑like protein
* ? NZ_CP034943 607566 =157 (0.840)9 (0.050)
+CGCTCCGGT
4/252 9.2 6.0% coding (409/1125 nt) EO946_RS03185 collagen‑like protein
?NZ_CP034943 = 607637 147 (0.780)coding (480/1125 nt) EO946_RS03185 collagen‑like protein
* ? NZ_CP034943 607515 =179 (0.950)10 (0.050)
+GGT
3/264 10.1 6.0% coding (358/1125 nt) EO946_RS03185 collagen‑like protein
?NZ_CP034943 = 607637 142 (0.760)coding (480/1125 nt) EO946_RS03185 collagen‑like protein
* ? NZ_CP034943 = 607256245 (1.310)4 (0.020) 3/264 10.1 1.7% coding (99/1125 nt) EO946_RS03185 collagen‑like protein
?NZ_CP034943 607428 = 210 (1.140)coding (271/1125 nt) EO946_RS03185 collagen‑like protein
* ? NZ_CP034943 = 1773981215 (1.150)3 (0.020) 3/268 10.2 1.5% coding (12490/15141 nt) EO946_RS09125 non‑ribosomal peptide synthetase
?NZ_CP034943 = 3717287 175 (0.940)intergenic (‑189/‑131) spsA/gerQ spore coat dTDP‑glycosyltransferase SpsA/spore coat protein GerQ
* ? NZ_CP034943 183537 =190 (1.010)3 (0.020) 3/270 10.2 1.4% coding (1488/1590 nt) EO946_RS01055 AraC family transcriptional regulator
?NZ_CP034943 = 530405 221 (1.180)coding (415/981 nt) EO946_RS02770 aldo/keto reductase