breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 39 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,979,2480TC50.0% ‑6.4 / 15.1 13noncoding (1553/2928 nt)EO946_RS1543023S ribosomal RNA
*NZ_CP0349433,285,5800AG45.2% 131.6 / inf 264I274T (ATA→ACA) EO946_RS16995IS3 family transposase
*NZ_CP0349433,285,6470CA45.2% 152.6 / inf 292V252L (GTA→TTA) EO946_RS16995IS3 family transposase
*NZ_CP0349433,285,5750TG39.9% 243.9 / 301.6 261N276H (AAT→CAT) EO946_RS16995IS3 family transposase
*NZ_CP0349433,285,5670CT39.1% 232.1 / inf 259L278L (CTG→CTAEO946_RS16995IS3 family transposase
*NZ_CP0349431,366,1150GC36.5% 134.3 / 32.2 101E205Q (GAA→CAA) ‡EO946_RS07135PepSY domain‑containing protein
*NZ_CP0349433,285,7130GA35.0% 183.6 / 146.7 140L230L (CTA→TTA) EO946_RS16995IS3 family transposase
*NZ_CP0349432,991,2270TG32.4% 204.4 / 41.6 142intergenic (+28/‑179)gbsR/bslAtranscriptional regulator GbsR/biofilm‑surface layer protein BslA
*NZ_CP03494317,8870GT31.6% 157.0 / 15.5 101E248D (GAG→GATdacAD‑alanyl‑D‑alanine carboxypeptidase
*NZ_CP034943634,0540CG31.5% 172.2 / 33.4 113E573Q (GAA→CAA) ‡EO946_RS03330ABC‑F family ATP‑binding cassette domain‑containing protein
*NZ_CP034943634,0640CG30.9% 223.8 / 27.5 121W569C (TGG→TGCEO946_RS03330ABC‑F family ATP‑binding cassette domain‑containing protein
*NZ_CP0349431,366,1170AC30.7% 139.9 / 24.0 101E205D (GAA→GAC) ‡EO946_RS07135PepSY domain‑containing protein
*NZ_CP0349432,991,2360CG28.4% 250.6 / 30.7 130intergenic (+37/‑170)gbsR/bslAtranscriptional regulator GbsR/biofilm‑surface layer protein BslA
*NZ_CP0349431,730,0960GC28.0% 131.9 / 12.1 75E173Q (GAA→CAA) rodZcell shape determination protein RodZ
*NZ_CP034943634,0580CG25.5% 203.7 / 13.0 113K571N (AAG→AACEO946_RS03330ABC‑F family ATP‑binding cassette domain‑containing protein
*NZ_CP0349432,270,0640GC24.8% 233.8 / 14.1 114L39V (CTA→GTA) prsWglutamic‑type intramembrane protease PrsW
*NZ_CP034943634,0520TG24.1% 203.5 / 15.2 113E573D (GAA→GAC) ‡EO946_RS03330ABC‑F family ATP‑binding cassette domain‑containing protein
*NZ_CP0349433,394,3670CG23.4% 412.3 / 22.1 172G178A (GGA→GCA) ppaXpyrophosphatase PpaX
*NZ_CP034943549,0850CA22.8% 332.0 / 31.3 171Q33K (CAA→AAA) EO946_RS02865PLP‑dependent aminotransferase family protein
*NZ_CP034943549,1440AC22.8% 253.3 / 11.0 136Q52H (CAA→CACEO946_RS02865PLP‑dependent aminotransferase family protein

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP034943 607695 =158 (0.900)22 (0.130)
+AGTAGCCCCT
16/248 4.4 10.7% coding (538/1125 nt) EO946_RS03185 collagen‑like protein
?NZ_CP034943 = 607846 241 (1.370)coding (689/1125 nt) EO946_RS03185 collagen‑like protein
* ? NZ_CP034943 = 607210238 (1.350)30 (0.170) 10/264 6.5 12.6% coding (53/1125 nt) EO946_RS03185 collagen‑like protein
?NZ_CP034943 607526 = 181 (1.040)coding (369/1125 nt) EO946_RS03185 collagen‑like protein
* ? NZ_CP034943 607659 =150 (0.850)9 (0.060)
+AGTAGCCCCT
5/248 8.3 4.7% coding (502/1125 nt) EO946_RS03185 collagen‑like protein
?NZ_CP034943 = 607846 242 (1.370)coding (689/1125 nt) EO946_RS03185 collagen‑like protein