mutation predictions | marginal predictions | summary statistics | genome diff | command line log
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breseq version 0.35.4 revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
| Marginal read alignment evidence (highest frequency 20 of 31 shown, sorted by frequency from high to low) | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP034943 | 3,285,647 | 0 | C | A | 41.7% | 189.2 / inf | 254 | V252L (GTA→TTA) | EO946_RS16995 | IS3 family transposase |
| * | NZ_CP034943 | 634,054 | 0 | C | G | 41.7% | 76.1 / 17.5 | 61 | E573Q (GAA→CAA) | EO946_RS03330 | ABC‑F family ATP‑binding cassette domain‑containing protein |
| * | NZ_CP034943 | 3,285,580 | 0 | A | G | 38.3% | 253.1 / 287.6 | 235 | I274T (ATA→ACA) | EO946_RS16995 | IS3 family transposase |
| * | NZ_CP034943 | 2,991,227 | 0 | T | G | 35.9% | 77.3 / 32.1 | 78 | intergenic (+28/‑179) | gbsR/bslA | transcriptional regulator GbsR/biofilm‑surface layer protein BslA |
| * | NZ_CP034943 | 3,285,567 | 0 | C | T | 35.5% | 249.6 / 234.4 | 211 | L278L (CTG→CTA) | EO946_RS16995 | IS3 family transposase |
| * | NZ_CP034943 | 3,285,713 | 0 | G | A | 35.3% | 146.1 / 124.4 | 116 | L230L (CTA→TTA) | EO946_RS16995 | IS3 family transposase |
| * | NZ_CP034943 | 2,991,236 | 0 | C | G | 34.5% | 96.7 / 23.5 | 69 | intergenic (+37/‑170) | gbsR/bslA | transcriptional regulator GbsR/biofilm‑surface layer protein BslA |
| * | NZ_CP034943 | 3,285,575 | 0 | T | G | 33.5% | 317.6 / 202.4 | 224 | N276H (AAT→CAT) | EO946_RS16995 | IS3 family transposase |
| * | NZ_CP034943 | 2,830,523 | 0 | C | G | 32.0% | 150.6 / 28.1 | 104 | L89V (CTT→GTT) | brnQ | branched‑chain amino acid transport system II carrier protein |
| * | NZ_CP034943 | 634,064 | 0 | C | G | 31.3% | 134.3 / 12.1 | 72 | W569C (TGG→TGC) | EO946_RS03330 | ABC‑F family ATP‑binding cassette domain‑containing protein |
| * | NZ_CP034943 | 1,366,109 | 0 | G | T | 31.2% | 145.3 / 12.4 | 96 | D203Y (GAT→TAT) | EO946_RS07135 | PepSY domain‑containing protein |
| * | NZ_CP034943 | 1,293,348 | 0 | G | T | 30.2% | 198.7 / 23.5 | 120 | V141L (GTA→TTA) | EO946_RS06740 | sigma‑70 family RNA polymerase sigma factor |
| * | NZ_CP034943 | 1,111,649 | 0 | C | A | 28.1% | 204.5 / 12.8 | 100 | P582T (CCG→ACG) | addB | helicase‑exonuclease AddAB subunit AddB |
| * | NZ_CP034943 | 3,446,970 | 0 | G | T | 28.0% | 149.7 / 29.1 | 107 | Q206K (CAA→AAA) | degU | two‑component system response regulator DegU |
| * | NZ_CP034943 | 3,446,980 | 0 | G | T | 25.7% | 183.6 / 13.8 | 105 | S202R (AGC→AGA) | degU | two‑component system response regulator DegU |
| * | NZ_CP034943 | 514,968 | 0 | G | T | 25.6% | 182.1 / 21.8 | 90 | V128L (GTA→TTA) | EO946_RS02645 | outer membrane lipoprotein carrier protein LolA |
| * | NZ_CP034943 | 595,177 | 0 | T | G | 25.2% | 206.6 / 15.4 | 112 | V21V (GTT→GTG) | EO946_RS03130 | MMPL family transporter |
| * | NZ_CP034943 | 2,387,026 | 0 | T | G | 24.1% | 211.4 / 10.7 | 108 | K367T (AAG→ACG) | EO946_RS12195 | acetyl‑CoA C‑acetyltransferase |
| * | NZ_CP034943 | 3,851,262 | 0 | C | A | 22.1% | 253.2 / 11.7 | 113 | W38L (TGG→TTG) | EO946_RS19820 | hypothetical protein |
| * | NZ_CP034943 | 3,219,823 | 0 | A | C | 22.1% | 256.4 / 12.9 | 127 | intergenic (+3/‑58) | oxdC/EO946_RS16675 | oxalate decarboxylase/regulatory YrvL family protein |
| Marginal new junction evidence (sorted from low to high skew) | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | NZ_CP034943 | = 607149 | 219 (1.600) | 5 (0.040) +TGCCGGGAGTCAT |
4/244 | 7.2 | 3.3% | intergenic (+515/‑9) | EO946_RS03180/EO946_RS03185 | glycosyltransferase/collagen‑like protein |
| ? | NZ_CP034943 | 607523 = | 107 (0.780) | coding (366/1125 nt) | EO946_RS03185 | collagen‑like protein | |||||
| * | ? | NZ_CP034943 | = 607486 | 115 (0.840) | 8 (0.060) +T |
4/268 | 7.6 | 6.9% | coding (329/1125 nt) | EO946_RS03185 | collagen‑like protein |
| ? | NZ_CP034943 | 607695 = | 103 (0.750) | coding (538/1125 nt) | EO946_RS03185 | collagen‑like protein | |||||
| * | ? | NZ_CP034943 | = 607474 | 118 (0.860) | 7 (0.060) | 3/242 | 7.7 | 6.5% | coding (317/1125 nt) | EO946_RS03185 | collagen‑like protein |
| ? | NZ_CP034943 | 607538 = | 96 (0.780) | coding (381/1125 nt) | EO946_RS03185 | collagen‑like protein | |||||
| * | ? | NZ_CP034943 | 2557054 = | 173 (1.260) | 5 (0.040) | 3/244 | 7.8 | 3.0% | coding (180/756 nt) | EO946_RS13225 | metal‑binding protein ZinT |
| ? | NZ_CP034943 | = 2557050 | 163 (1.320) | coding (184/756 nt) | EO946_RS13225 | metal‑binding protein ZinT | |||||
| * | ? | NZ_CP034943 | 767776 = | 147 (1.070) | 3 (0.020) | 3/244 | 7.8 | 2.1% | coding (189/645 nt) | EO946_RS03930 | hypothetical protein |
| ? | NZ_CP034943 | = 767772 | 142 (1.150) | coding (193/645 nt) | EO946_RS03930 | hypothetical protein | |||||
| * | ? | NZ_CP034943 | 607179 = | 239 (1.750) | 7 (0.050) | 3/254 | 7.9 | 4.0% | coding (22/1125 nt) | EO946_RS03185 | collagen‑like protein |
| ? | NZ_CP034943 | = 607466 | 114 (0.890) | coding (309/1125 nt) | EO946_RS03185 | collagen‑like protein | |||||
| * | ? | NZ_CP034943 | = 2408000 | 91 (0.660) | 3 (0.020) | 3/270 | 8.2 | 3.3% | coding (493/621 nt) | spoIIIAF | stage III sporulation protein AF |
| ? | NZ_CP034943 | 2408004 = | 87 (0.640) | coding (489/621 nt) | spoIIIAF | stage III sporulation protein AF | |||||