breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 30 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24401,123,2380CA55.0% 9.6 / 32.8 41intergenic (‑240/+64)gcvH_1/CJ019_01062Glycine cleavage system H protein/hypothetical protein
*KT24405,147,1330TG52.4% ‑2.5 / 10.1 21intergenic (+187/+30)ybdG_2/bioPMiniconductance mechanosensitive channel YbdG/Biotin transporter
*KT24402,856,8860TG43.3% 0.6 / 10.7 30S297A (TCG→GCG) hdfR_4HTH‑type transcriptional regulator HdfR
*KT24403,026,5900AC39.1% 39.8 / 12.2 46Q19P (CAG→CCG) ‡CJ019_02841hypothetical protein
*KT24404,350,3600TG38.5% 56.0 / 11.7 53R28R (CGT→CGGCJ019_04090hypothetical protein
*KT24403,026,5910GC37.8% 47.6 / 10.9 46Q19H (CAG→CAC) ‡CJ019_02841hypothetical protein
*KT24404,427,1180GC36.8% 44.4 / 26.5 38intergenic (‑29/‑89)CJ019_04200/CJ019_04201hypothetical protein/hypothetical protein
*KT24404,350,3630CG30.6% 84.2 / 11.8 53F29L (TTC→TTGCJ019_04090hypothetical protein
*KT24401,278,0860CG30.4% 64.8 / 16.9 46G63G (GGC→GGGompA_1Outer membrane protein A
*KT24405,361,6410AC30.4% 84.8 / 10.6 69R145R (CGT→CGGCJ019_05046hypothetical protein
*KT24405,384,2760CG30.2% 104.6 / 12.3 53intergenic (+539/‑319)CJ019_05064/CJ019_05067putative protein/hypothetical protein
*KT2440151,0100CG30.0% 55.5 / 13.6 41D44H (GAC→CAC) CJ019_00157hypothetical protein
*KT2440434,3760G.30.0% 68.2 / 30.8 30coding (207/1173 nt)bioF8‑amino‑7‑oxononanoate synthase
*KT24404,427,1080CA29.7% 53.7 / 14.3 37intergenic (‑19/‑99)CJ019_04200/CJ019_04201hypothetical protein/hypothetical protein
*KT2440237,8820AG29.5% 54.3 / 16.4 53T171A (ACT→GCT) CJ019_00211hypothetical protein
*KT24401,278,0950CG28.3% 87.0 / 10.5 47G66G (GGC→GGGompA_1Outer membrane protein A
*KT2440411,3390CA26.7% 165.2 / 14.9 76F24L (TTC→TTAkdkA_13‑deoxy‑D‑manno‑octulosonic acid kinase
*KT24403,138,3670TG26.3% 95.4 / 10.4 57F240V (TTT→GTT) rbsC_2Ribose import permease protein RbsC
*KT24401,115,4440TG26.0% 88.8 / 10.2 50L242R (CTT→CGT) lptGLipopolysaccharide export system permeaseprotein LptG
*KT24402,582,2360CG25.9% 110.0 / 11.3 54R848R (CGC→CGGCJ019_02433hypothetical protein

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? KT2440 4963351 =66 (0.890)6 (0.090) 6/232 5.9 9.0% coding (715/2064 nt) CJ019_04659 hypothetical protein
?KT2440 = 4963347 62 (0.930)coding (719/2064 nt) CJ019_04659 hypothetical protein
* ? KT2440 3965421 =92 (1.250)40 (0.820)
+44 bp
3/170 5.9 41.8% intergenic (+159/‑221) CJ019_03744/CJ019_00572 IS66 family transposase ISPpu14/IS110 family transposase ISPpu10
?KT2440 5821636 = 77 (1.040)intergenic (+3/+196) mtgA/rpoH Biosynthetic peptidoglycan transglycosylase/RNA polymerase sigma factor RpoH