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breseq version 0.35.4 revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Marginal read alignment evidence (highest frequency 20 of 30 shown, sorted by frequency from high to low) | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | KT2440 | 1,123,238 | 0 | C | A | 55.0% | 9.6 / 32.8 | 41 | intergenic (‑240/+64) | gcvH_1/CJ019_01062 | Glycine cleavage system H protein/hypothetical protein |
* | KT2440 | 5,147,133 | 0 | T | G | 52.4% | ‑2.5 / 10.1 | 21 | intergenic (+187/+30) | ybdG_2/bioP | Miniconductance mechanosensitive channel YbdG/Biotin transporter |
* | KT2440 | 2,856,886 | 0 | T | G | 43.3% | 0.6 / 10.7 | 30 | S297A (TCG→GCG) | hdfR_4 | HTH‑type transcriptional regulator HdfR |
* | KT2440 | 3,026,590 | 0 | A | C | 39.1% | 39.8 / 12.2 | 46 | Q19P (CAG→CCG) ‡ | CJ019_02841 | hypothetical protein |
* | KT2440 | 4,350,360 | 0 | T | G | 38.5% | 56.0 / 11.7 | 53 | R28R (CGT→CGG) | CJ019_04090 | hypothetical protein |
* | KT2440 | 3,026,591 | 0 | G | C | 37.8% | 47.6 / 10.9 | 46 | Q19H (CAG→CAC) ‡ | CJ019_02841 | hypothetical protein |
* | KT2440 | 4,427,118 | 0 | G | C | 36.8% | 44.4 / 26.5 | 38 | intergenic (‑29/‑89) | CJ019_04200/CJ019_04201 | hypothetical protein/hypothetical protein |
* | KT2440 | 4,350,363 | 0 | C | G | 30.6% | 84.2 / 11.8 | 53 | F29L (TTC→TTG) | CJ019_04090 | hypothetical protein |
* | KT2440 | 1,278,086 | 0 | C | G | 30.4% | 64.8 / 16.9 | 46 | G63G (GGC→GGG) | ompA_1 | Outer membrane protein A |
* | KT2440 | 5,361,641 | 0 | A | C | 30.4% | 84.8 / 10.6 | 69 | R145R (CGT→CGG) | CJ019_05046 | hypothetical protein |
* | KT2440 | 5,384,276 | 0 | C | G | 30.2% | 104.6 / 12.3 | 53 | intergenic (+539/‑319) | CJ019_05064/CJ019_05067 | putative protein/hypothetical protein |
* | KT2440 | 151,010 | 0 | C | G | 30.0% | 55.5 / 13.6 | 41 | D44H (GAC→CAC) | CJ019_00157 | hypothetical protein |
* | KT2440 | 434,376 | 0 | G | . | 30.0% | 68.2 / 30.8 | 30 | coding (207/1173 nt) | bioF | 8‑amino‑7‑oxononanoate synthase |
* | KT2440 | 4,427,108 | 0 | C | A | 29.7% | 53.7 / 14.3 | 37 | intergenic (‑19/‑99) | CJ019_04200/CJ019_04201 | hypothetical protein/hypothetical protein |
* | KT2440 | 237,882 | 0 | A | G | 29.5% | 54.3 / 16.4 | 53 | T171A (ACT→GCT) | CJ019_00211 | hypothetical protein |
* | KT2440 | 1,278,095 | 0 | C | G | 28.3% | 87.0 / 10.5 | 47 | G66G (GGC→GGG) | ompA_1 | Outer membrane protein A |
* | KT2440 | 411,339 | 0 | C | A | 26.7% | 165.2 / 14.9 | 76 | F24L (TTC→TTA) | kdkA_1 | 3‑deoxy‑D‑manno‑octulosonic acid kinase |
* | KT2440 | 3,138,367 | 0 | T | G | 26.3% | 95.4 / 10.4 | 57 | F240V (TTT→GTT) | rbsC_2 | Ribose import permease protein RbsC |
* | KT2440 | 1,115,444 | 0 | T | G | 26.0% | 88.8 / 10.2 | 50 | L242R (CTT→CGT) | lptG | Lipopolysaccharide export system permeaseprotein LptG |
* | KT2440 | 2,582,236 | 0 | C | G | 25.9% | 110.0 / 11.3 | 54 | R848R (CGC→CGG) | CJ019_02433 | hypothetical protein |
Marginal new junction evidence (sorted from low to high skew) | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | KT2440 | 4963351 = | 66 (0.890) | 6 (0.090) | 6/232 | 5.9 | 9.0% | coding (715/2064 nt) | CJ019_04659 | hypothetical protein |
? | KT2440 | = 4963347 | 62 (0.930) | coding (719/2064 nt) | CJ019_04659 | hypothetical protein | |||||
* | ? | KT2440 | 3965421 = | 92 (1.250) | 40 (0.820) +44 bp |
3/170 | 5.9 | 41.8% | intergenic (+159/‑221) | CJ019_03744/CJ019_00572 | IS66 family transposase ISPpu14/IS110 family transposase ISPpu10 |
? | KT2440 | 5821636 = | 77 (1.040) | intergenic (+3/+196) | mtgA/rpoH | Biosynthetic peptidoglycan transglycosylase/RNA polymerase sigma factor RpoH |