breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 34 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,285,6470CA41.6% 202.4 / inf 298V252L (GTA→TTA) EO946_RS16995IS3 family transposase
*NZ_CP0349433,285,5800AG40.0% 256.5 / inf 286I274T (ATA→ACA) EO946_RS16995IS3 family transposase
*NZ_CP0349433,285,5750TG36.4% 348.2 / 308.4 298N276H (AAT→CAT) EO946_RS16995IS3 family transposase
*NZ_CP0349433,285,5670CT35.2% 350.5 / inf 298L278L (CTG→CTAEO946_RS16995IS3 family transposase
*NZ_CP0349432,991,2270TG32.8% 179.9 / 36.3 123intergenic (+28/‑179)gbsR/bslAtranscriptional regulator GbsR/biofilm‑surface layer protein BslA
*NZ_CP0349432,991,2360CG31.2% 183.5 / 29.3 116intergenic (+37/‑170)gbsR/bslAtranscriptional regulator GbsR/biofilm‑surface layer protein BslA
*NZ_CP0349432,991,2520CA29.8% 166.2 / 19.5 105intergenic (+53/‑154)gbsR/bslAtranscriptional regulator GbsR/biofilm‑surface layer protein BslA
*NZ_CP0349433,285,7130GA29.8% 228.1 / 121.7 141L230L (CTA→TTA) EO946_RS16995IS3 family transposase
*NZ_CP034943634,0540CG28.0% 233.9 / 22.2 121E573Q (GAA→CAA) EO946_RS03330ABC‑F family ATP‑binding cassette domain‑containing protein
*NZ_CP0349431,730,0930GC27.9% 174.4 / 20.2 106E172Q (GAA→CAA) rodZcell shape determination protein RodZ
*NZ_CP0349431,730,0750GC27.8% 228.0 / 25.6 126E166Q (GAA→CAA) rodZcell shape determination protein RodZ
*NZ_CP0349432,991,2450GT27.6% 170.2 / 17.6 116intergenic (+46/‑161)gbsR/bslAtranscriptional regulator GbsR/biofilm‑surface layer protein BslA
*NZ_CP0349432,270,0640GC26.8% 225.7 / 19.5 134L39V (CTA→GTA) prsWglutamic‑type intramembrane protease PrsW
*NZ_CP0349432,270,0520GC26.3% 232.7 / 23.3 136L43V (CTT→GTT) prsWglutamic‑type intramembrane protease PrsW
*NZ_CP034943549,1440AC26.0% 262.8 / 27.4 150Q52H (CAA→CACEO946_RS02865PLP‑dependent aminotransferase family protein
*NZ_CP0349432,270,0430GT25.4% 210.3 / 19.1 138P46T (CCC→ACC) prsWglutamic‑type intramembrane protease PrsW
*NZ_CP034943718,2990CT25.1% 341.9 / 129.1 180intergenic (+325/‑666)rlmD/EO946_RS0370523S rRNA (uracil(1939)‑C(5))‑methyltransferase RlmD/type I restriction‑modification system subunit M
*NZ_CP0349433,394,3780CG25.0% 330.5 / 19.0 166G174G (GGG→GGCppaXpyrophosphatase PpaX
*NZ_CP0349431,366,1170AC24.3% 245.8 / 15.4 137E205D (GAA→GACEO946_RS07135PepSY domain‑containing protein
*NZ_CP0349433,557,3000CA24.2% 377.7 / 18.3 201V886V (GTG→GTTEO946_RS18295SpaA isopeptide‑forming pilin‑related protein

Marginal new junction evidence (lowest skew 10 of 17 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP034943 = 619275139 (0.690)48 (0.240) 22/266 3.9 25.6% coding (1271/1398 nt) gmuD 6‑phospho‑beta‑glucosidase GmuD
?NZ_CP034943 619290 = 142 (0.720)coding (1286/1398 nt) gmuD 6‑phospho‑beta‑glucosidase GmuD
* ? NZ_CP034943 607677 =149 (0.740)40 (0.200)
+C
15/268 5.5 17.8% coding (520/1125 nt) EO946_RS03185 collagen‑like protein
?NZ_CP034943 = 607837 222 (1.110)coding (680/1125 nt) EO946_RS03185 collagen‑like protein
* ? NZ_CP034943 2557054 =258 (1.290)6 (0.030) 6/244 8.1 2.4% coding (180/756 nt) EO946_RS13225 metal‑binding protein ZinT
?NZ_CP034943 = 2557050 247 (1.370)coding (184/756 nt) EO946_RS13225 metal‑binding protein ZinT
* ? NZ_CP034943 = 607210321 (1.600)18 (0.090) 7/266 8.1 6.7% coding (53/1125 nt) EO946_RS03185 collagen‑like protein
?NZ_CP034943 607526 = 182 (0.920)coding (369/1125 nt) EO946_RS03185 collagen‑like protein
* ? NZ_CP034943 = 607210321 (1.600)15 (0.080) 7/266 8.1 5.4% coding (53/1125 nt) EO946_RS03185 collagen‑like protein
?NZ_CP034943 607481 = 213 (1.080)coding (324/1125 nt) EO946_RS03185 collagen‑like protein
* ? NZ_CP034943 607179 =310 (1.550)12 (0.060)
+CGCTCCC
6/256 8.4 4.4% coding (22/1125 nt) EO946_RS03185 collagen‑like protein
?NZ_CP034943 = 607837 238 (1.190)coding (680/1125 nt) EO946_RS03185 collagen‑like protein
* ? NZ_CP034943 = 607222306 (1.530)13 (0.070)
+TACC
6/262 8.5 5.7% coding (65/1125 nt) EO946_RS03185 collagen‑like protein
?NZ_CP034943 607587 = 140 (0.700)coding (430/1125 nt) EO946_RS03185 collagen‑like protein
* ? NZ_CP034943 = 607225295 (1.470)4 (0.020)
+26 bp
4/218 8.6 2.2% coding (68/1125 nt) EO946_RS03185 collagen‑like protein
?NZ_CP034943 607675 = 149 (0.740)coding (518/1125 nt) EO946_RS03185 collagen‑like protein
* ? NZ_CP034943 607479 =224 (1.120)3 (0.020) 3/218 9.2 1.9% coding (322/1125 nt) EO946_RS03185 collagen‑like protein
?NZ_CP034943 = 607604 133 (0.820)coding (447/1125 nt) EO946_RS03185 collagen‑like protein
* ? NZ_CP034943 = 607409224 (1.120)6 (0.030) 4/260 9.6 2.7% coding (252/1125 nt) EO946_RS03185 collagen‑like protein
?NZ_CP034943 607419 = 212 (1.100)coding (262/1125 nt) EO946_RS03185 collagen‑like protein