breseq version 0.35.4 revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Predicted mutations | ||||||
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evidence | position | mutation | freq | annotation | gene | description |
RA | 52,254 | T→C | 5.7% | intergenic (+15/‑196) | prtG → / → EO946_RS00280 | sporulation‑specific protease PrtG/Veg family protein |
RA | 52,263 | T→G | 5.7% | intergenic (+24/‑187) | prtG → / → EO946_RS00280 | sporulation‑specific protease PrtG/Veg family protein |
RA | 112,591 | A→T | 9.2% | intergenic (+269/‑35) | gltX → / → cysE | glutamate‑‑tRNA ligase/serine O‑acetyltransferase |
RA | 177,053 | C→A | 23.2% | intergenic (+226/‑46) | EO946_RS01020 → / → EO946_RS01025 | alcohol dehydrogenase/SulP family inorganic anion transporter |
RA | 177,101 | G→T | 6.5% | M1F (TTG→TTT) † | EO946_RS01025 → | SulP family inorganic anion transporter |
RA | 240,174 | T→G | 8.7% | N120H (AAC→CAC) | EO946_RS01360 ← | hypothetical protein |
RA | 240,207 | G→T | 12.9% | Q109K (CAA→AAA) | EO946_RS01360 ← | hypothetical protein |
RA | 443,396 | G→T | 6.4% | G261V (GGA→GTA) | gdh → | glucose 1‑dehydrogenase |
RA | 607,359 | A→C | 16.1% | T68P (ACC→CCC) | EO946_RS03185 → | collagen‑like protein |
RA | 607,413 | A→C | 9.4% | T86P (ACC→CCC) | EO946_RS03185 → | collagen‑like protein |
RA | 607,671 | C→G | 6.5% | P172A (CCA→GCA) ‡ | EO946_RS03185 → | collagen‑like protein |
RA | 607,673 | A→G | 5.8% | P172P (CCA→CCG) ‡ | EO946_RS03185 → | collagen‑like protein |
RA | 607,674 | A→C | 6.8% | T173P (ACC→CCC) | EO946_RS03185 → | collagen‑like protein |
RA | 607,682 | G→C | 14.6% | A175A (GCG→GCC) | EO946_RS03185 → | collagen‑like protein |
RA | 607,688 | A→G | 12.3% | G177G (GGA→GGG) | EO946_RS03185 → | collagen‑like protein |
RA | 607,700 | A→C | 27.4% | E181D (GAA→GAC) | EO946_RS03185 → | collagen‑like protein |
RA | 607,702 | C→T | 19.9% | A182V (GCC→GTC) | EO946_RS03185 → | collagen‑like protein |
RA | 607,838 | A→G | 8.8% | T227T (ACA→ACG) | EO946_RS03185 → | collagen‑like protein |
RA | 704,348 | Δ1 bp | 100% | intergenic (+170/‑14) | EO946_RS03650 → / → EO946_RS03655 | CamS family sex pheromone protein/phosphotransferase enzyme family protein |
RA | 755,531 | A→C | 11.6% | Q22H (CAA→CAC) | EO946_RS03880 → | rhamnogalacturonan acetylesterase |
RA | 767,777 | C→G | 5.3% | G63A (GGC→GCC) | EO946_RS03930 ← | hypothetical protein |
RA | 828,787 | G→A | 100% | E77K (GAA→AAA) | treC → | alpha,alpha‑phosphotrehalase |
RA | 886,754 | A→C | 10.4% | V45G (GTT→GGT) | EO946_RS04490 ← | MFS transporter |
RA | 1,035,865 | G→T | 9.8% | C200F (TGC→TTC) | EO946_RS05350 → | ABC transporter ATP‑binding protein |
RA | 1,040,035 | G→C | 6.0% | D429H (GAT→CAT) | EO946_RS05365 → | AAA family ATPase |
RA | 1,111,632 | T→G | 8.4% | L576R (CTT→CGT) | addB → | helicase‑exonuclease AddAB subunit AddB |
RA | 1,111,643 | C→A | 9.6% | L580I (CTA→ATA) | addB → | helicase‑exonuclease AddAB subunit AddB |
RA | 1,152,026 | G→C | 7.8% | G59G (GGC→GGG) | EO946_RS05920 ← | GNAT family N‑acetyltransferase |
RA | 1,263,953 | G→T | 9.7% | I77I (ATC→ATA) | fetB ← | iron export ABC transporter permease subunit FetB |
RA | 1,514,854 | T→G | 9.1% | intergenic (+58/+1) | EO946_RS07915 → / ← EO946_RS07920 | YlaH‑like family protein/YlaI family protein |
RA | 1,514,935 | G→T | 5.5% | Q44K (CAA→AAA) | EO946_RS07920 ← | YlaI family protein |
RA | 1,936,618 | G→C | 6.4% | L3545V (CTG→GTG) | EO946_RS09735 ← | non‑ribosomal peptide synthetase |
RA | 1,950,238 | G→A | 6.5% | I2995I (ATC→ATT) | EO946_RS09740 ← | non‑ribosomal peptide synthetase |
RA | 1,968,161 | G→T | 17.6% | D78Y (GAT→TAT) | EO946_RS09780 → | hypothetical protein |
RA | 2,214,528 | G→T | 5.6% | Q57K (CAA→AAA) | nth ← | endonuclease III |
RA | 2,350,852 | G→T | 5.7% | pseudogene (929/1182 nt) | EO946_RS12020 ← | IS3 family transposase |
RA | 2,379,799 | T→G | 6.3% | K382T (AAG→ACG) | EO946_RS12160 ← | sigma‑54‑dependent Fis family transcriptional regulator |
RA | 2,491,058 | T→G | 6.0% | E271D (GAA→GAC) | floA ← | flotillin‑like protein FloA |
RA | 2,502,212 | T→G | 6.4% | N284H (AAC→CAC) | hrcA ← | heat‑inducible transcriptional repressor HrcA |
RA | 2,680,491 | A→C | 6.3% | F387V (TTC→GTC) | EO946_RS13875 ← | LysM peptidoglycan‑binding domain‑containing protein |
RA | 2,812,790 | T→G | 8.4% | E199D (GAA→GAC) | EO946_RS14520 ← | SDR family oxidoreductase |
RA | 2,901,671 | A→C | 14.6% | K411T (AAA→ACA) | EO946_RS14945 → | extracellular solute‑binding protein |
RA | 2,978,569 | C→T | 14.9% | noncoding (2232/2928 nt) | EO946_RS15430 ← | 23S ribosomal RNA |
RA | 2,979,834 | C→T | 10.8% | noncoding (967/2928 nt) | EO946_RS15430 ← | 23S ribosomal RNA |
RA | 2,980,160 | G→A | 29.2% | noncoding (641/2928 nt) | EO946_RS15430 ← | 23S ribosomal RNA |
RA | 2,982,236 | T→C | 46.2% | noncoding (284/1550 nt) | EO946_RS15435 ← | 16S ribosomal RNA |
RA | 2,991,245 | G→T | 18.0% | intergenic (+46/‑161) | gbsR → / → bslA | transcriptional regulator GbsR/biofilm‑surface layer protein BslA |
RA | 3,217,687 | T→C | 7.2% | K166K (AAA→AAG) | sigO ← | RNA polymerase sigma factor SigO |
RA | 3,217,702 | A→C | 7.3% | L161L (CTT→CTG) | sigO ← | RNA polymerase sigma factor SigO |
RA | 3,217,708 | T→C | 6.8% | K159K (AAA→AAG) | sigO ← | RNA polymerase sigma factor SigO |
RA | 3,219,740 | C→A | 7.9% | H360N (CAT→AAT) | oxdC → | oxalate decarboxylase |
RA | 3,219,794 | G→T | 7.3% | V378F (GTT→TTT) | oxdC → | oxalate decarboxylase |
RA | 3,247,065 | C→T | 100% | intergenic (‑11/+110) | bdbD ← / ← cadA | disulfide bond formation protein BdbD/cadmium‑translocating P‑type ATPase |
RA | 3,280,377 | G→T | 7.0% | Q115K (CAA→AAA) | EO946_RS16965 ← | lantibiotic dehydratase |
RA | 3,285,567 | C→T | 30.7% | L278L (CTG→CTA) | EO946_RS16995 ← | IS3 family transposase |
RA | 3,285,575 | T→G | 31.4% | N276H (AAT→CAT) | EO946_RS16995 ← | IS3 family transposase |
RA | 3,285,580 | A→G | 34.1% | I274T (ATA→ACA) | EO946_RS16995 ← | IS3 family transposase |
RA | 3,285,647 | C→A | 34.0% | V252L (GTA→TTA) | EO946_RS16995 ← | IS3 family transposase |
RA | 3,285,713 | G→A | 24.4% | L230L (CTA→TTA) | EO946_RS16995 ← | IS3 family transposase |
RA | 3,319,761 | T→G | 7.2% | pseudogene (544/1292 nt) | EO946_RS17170 ← | arabinogalactan endo‑beta‑1,4‑galactanase |
RA | 3,369,578 | C→A | 16.4% | G110W (GGG→TGG) | EO946_RS17410 ← | HAMP domain‑containing histidine kinase |
RA | 3,447,038 | T→G | 11.2% | N183T (AAC→ACC) | degU ← | two‑component system response regulator DegU |
RA | 3,496,387 | G→T | 9.2% | S347R (AGC→AGA) | EO946_RS17995 ← | glucosaminidase domain‑containing protein |
RA | 3,496,423 | G→T | 8.8% | F335L (TTC→TTA) | EO946_RS17995 ← | glucosaminidase domain‑containing protein |
RA | 3,584,291 | G→T | 5.5% | S324S (TCG→TCT) | EO946_RS18420 → | N‑acetylneuraminate synthase family protein |
RA | 3,584,300 | G→C | 7.0% | Q327H (CAG→CAC) | EO946_RS18420 → | N‑acetylneuraminate synthase family protein |
RA | 3,584,316 | G→C | 5.6% | E333Q (GAA→CAA) | EO946_RS18420 → | N‑acetylneuraminate synthase family protein |
RA | 3,719,864 | T→G | 7.0% | S79R (AGC→CGC) | EO946_RS19145 ← | YwdI family protein |
RA | 3,769,576 | T→G | 8.3% | I281S (ATT→AGT) | sacX → | SacY negative regulator SacX |
RA | 3,851,237 | G→T | 9.0% | I46I (ATC→ATA) | EO946_RS19820 ← | hypothetical protein |
RA | 3,851,254 | G→C | 5.1% | L41V (CTT→GTT) | EO946_RS19820 ← | hypothetical protein |
RA | 3,906,067 | A→C | 14.3% | I42S (ATT→AGT) | EO946_RS20100 ← | DUF3255 family protein |
RA | 3,996,857 | C→A | 7.1% | intergenic (‑163/+43) | purA ← / ← EO946_RS20530 | adenylosuccinate synthase/VOC family protein |
RA | 3,996,927 | C→A | 7.2% | M131I (ATG→ATT) | EO946_RS20530 ← | VOC family protein |
RA | 4,022,597 | G→T | 5.5% | V607F (GTT→TTT) | EO946_RS20675 → | thioredoxin domain‑containing protein |
Unassigned new junction evidence | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | NZ_CP034943 | = 91969 | NA (NA) | 11 (0.040) | 5/250 | NT | NA | intergenic (+131/‑39) | EO946_RS00485/EO946_RS00490 | 16S ribosomal RNA/23S ribosomal RNA |
? | NZ_CP034943 | 91973 = | NA (NA) | intergenic (+135/‑35) | EO946_RS00485/EO946_RS00490 | 16S ribosomal RNA/23S ribosomal RNA |