breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 44,293 Δ1 bp 100% intergenic (+124/‑367) oprD_1 → / → czcR_2 Porin D/Transcriptional activator protein CzcR
RA 120,975 Δ1 bp 100% intergenic (+272/+100) polA → / ← engB DNA polymerase I/putative GTP‑binding protein EngB
RA 169,705 A→C 20.1% intergenic (+669/+3018) PP_RS08750 → / ← fhuA_1 tRNA‑Val/Ferrichrome outer membrane transporter/phagereceptor
RA 169,760 A→C 17.6% intergenic (+724/+2963) PP_RS08750 → / ← fhuA_1 tRNA‑Val/Ferrichrome outer membrane transporter/phagereceptor
RA 169,792 G→A 14.0% intergenic (+756/+2931) PP_RS08750 → / ← fhuA_1 tRNA‑Val/Ferrichrome outer membrane transporter/phagereceptor
RA 169,811 C→A 12.6% intergenic (+775/+2912) PP_RS08750 → / ← fhuA_1 tRNA‑Val/Ferrichrome outer membrane transporter/phagereceptor
RA 169,812 T→C 9.8% intergenic (+776/+2911) PP_RS08750 → / ← fhuA_1 tRNA‑Val/Ferrichrome outer membrane transporter/phagereceptor
RA 169,813 A→G 11.7% intergenic (+777/+2910) PP_RS08750 → / ← fhuA_1 tRNA‑Val/Ferrichrome outer membrane transporter/phagereceptor
RA 169,817 T→A 11.7% intergenic (+781/+2906) PP_RS08750 → / ← fhuA_1 tRNA‑Val/Ferrichrome outer membrane transporter/phagereceptor
RA 169,819 T→G 11.7% intergenic (+783/+2904) PP_RS08750 → / ← fhuA_1 tRNA‑Val/Ferrichrome outer membrane transporter/phagereceptor
RA 169,820 T→C 11.7% intergenic (+784/+2903) PP_RS08750 → / ← fhuA_1 tRNA‑Val/Ferrichrome outer membrane transporter/phagereceptor
RA 169,821 A→G 12.1% intergenic (+785/+2902) PP_RS08750 → / ← fhuA_1 tRNA‑Val/Ferrichrome outer membrane transporter/phagereceptor
RA 169,822 G→T 12.1% intergenic (+786/+2901) PP_RS08750 → / ← fhuA_1 tRNA‑Val/Ferrichrome outer membrane transporter/phagereceptor
RA 179,484 T→C 100% C316R (TGC→CGC)  CJ019_00182 → hypothetical protein
RA 211,447 G→A 6.2% G8769D (GGC→GAC)  CJ019_00185 → hypothetical protein
RA 214,309 G→A 5.5% D12N (GAC→AAC)  tauD_1 → Alpha‑ketoglutarate‑dependent taurinedioxygenase
RA 346,909 C→A 8.5% L157L (CTG→CTT btuD_1 ← Vitamin B12 import ATP‑binding protein BtuD
RA 535,779 G→T 10.0% P323P (CCG→CCT rpoC → DNA‑directed RNA polymerase subunit beta'
RA 535,785 G→A 9.1% K325K (AAG→AAA rpoC → DNA‑directed RNA polymerase subunit beta'
RA 535,788 C→T 9.1% S326S (TCC→TCT rpoC → DNA‑directed RNA polymerase subunit beta'
RA 535,789 C→T 9.1% L327L (CTG→TTG)  rpoC → DNA‑directed RNA polymerase subunit beta'
RA 535,812 G→A 10.3% K334K (AAG→AAA rpoC → DNA‑directed RNA polymerase subunit beta'
RA 535,815 A→G 10.2% Q335Q (CAA→CAG rpoC → DNA‑directed RNA polymerase subunit beta'
RA 740,674 T→G 29.2% L38R (CTG→CGG)  CJ019_00704 → hypothetical protein
RA 759,412 C→G 31.5% intergenic (+21/‑147) mmuM_1 → / → CJ019_00729 Homocysteine S‑methyltransferase/L‑methionine gamma‑lyase
RA 970,158 C→T 7.1% R20R (CGC→CGT cysE → Serine acetyltransferase
RA 1,311,107 Δ1 bp 27.0% coding (1472/1989 nt) aaeB_2 → p‑hydroxybenzoic acid efflux pump subunit AaeB
RA 1,311,108 Δ1 bp 26.6% coding (1473/1989 nt) aaeB_2 → p‑hydroxybenzoic acid efflux pump subunit AaeB
RA 1,311,561 Δ1 bp 12.1% coding (1926/1989 nt) aaeB_2 → p‑hydroxybenzoic acid efflux pump subunit AaeB
RA 1,312,061 C→T 8.9% R78C (CGC→TGC)  aaeA_2 → p‑hydroxybenzoic acid efflux pump subunit AaeA
RA 1,466,816 C→A 7.2% A153A (GCG→GCT algD ← GDP‑mannose 6‑dehydrogenase
RA 1,550,590 C→T 5.7% K213K (AAG→AAA CJ019_01464 ← hypothetical protein
RA 1,628,160 G→A 6.2% G118D (GGC→GAC)  era → GTPase Era
RA 1,630,560 T→A 19.1% V412V (GTA→GTT czcS ← Sensor protein CzcS
RA 1,686,855 C→G 27.4% L90V (CTG→GTG)  CJ019_01584 → hypothetical protein
RA 1,699,121 C→T 5.6% R44C (CGT→TGT)  CJ019_01595 → hypothetical protein
RA 1,721,874 A→C 29.8% W97G (TGG→GGG)  CJ019_01617 ← hypothetical protein
RA 1,812,261 C→A 8.4% T150T (ACC→ACA rpoS → RNA polymerase sigma factor RpoS
RA 1,834,850 T→G 25.9% D57A (GAC→GCC)  ldhA ← D‑lactate dehydrogenase
RA 1,834,908 T→G 29.7% T38P (ACC→CCC)  ldhA ← D‑lactate dehydrogenase
RA 1,838,093 C→T 8.3% D31D (GAC→GAT cseB → Transcriptional regulatory protein CseB
RA 2,074,770 G→T 7.7% intergenic (‑88/+53) ohrA ← / ← ohrR Organic hydroperoxide resistance protein OhrA/Organic hydroperoxide resistance transcriptional regulator
RA 2,094,790 A→C 21.4% V192G (GTG→GGG)  CJ019_01995 ← hypothetical protein
RA 2,094,808 A→C 49.1% L186R (CTG→CGG)  CJ019_01995 ← hypothetical protein
RA 2,094,817 T→G 38.0% Q183P (CAG→CCG)  CJ019_01995 ← hypothetical protein
RA 2,106,773 T→G 15.9% E190D (GAA→GAC CJ019_02004 ← hypothetical protein
RA 2,122,874 T→G 9.6% Q794H (CAA→CAC fimD_1 ← Outer membrane usher protein FimD
RA 2,133,554 T→G 10.6% L78R (CTG→CGG)  comEC → ComE operon protein 3
JC 2,191,046 +CTGAAAATATCTCTTAT 100% intergenic (‑1/‑313) PP_RS10045 ← / → purU_3 LysR family transcriptional regulator/Formyltetrahydrofolate deformylase
RA 2,348,245 T→G 15.3% E268A (GAG→GCG)  oprJ_1 ← Outer membrane protein OprJ
RA 2,386,955 T→G 26.1% intergenic (+41/+65) CJ019_02252 → / ← dacC_1 hypothetical protein/D‑alanyl‑D‑alanine carboxypeptidase DacC
RA 2,390,993 T→G 17.6% E566D (GAA→GAC bvgS ← Virulence sensor protein BvgS
RA 2,525,483 T→A 9.3% V52E (GTA→GAA)  CJ019_02380 → hypothetical protein
RA 2,647,706 T→A 8.7% Y119N (TAT→AAT)  cysB → HTH‑type transcriptional regulator CysB
RA 2,725,622 T→G 29.4% V640G (GTG→GGG)  polB → DNA polymerase II
RA 3,365,199 A→C 6.2% intergenic (‑87/‑32) csoR ← / → CJ019_03165 Copper‑sensing transcriptional repressor CsoR/hypothetical protein
RA 3,798,113 C→T 7.4% R29Q (CGG→CAG)  tmk_3 ← Thymidylate kinase
RA 3,803,123 G→A 8.7% R67H (CGC→CAC)  cynR_5 → HTH‑type transcriptional regulator CynR
RA 3,887,510 G→A 7.4% R393C (CGC→TGC)  CJ019_03679 ← hypothetical protein
RA 3,935,250 C→T 9.5% E137K (GAA→AAA)  CJ019_03720 ← hypothetical protein
RA 4,119,178 G→A 7.1% A94A (GCC→GCT CJ019_03877 ← hypothetical protein
RA 4,184,142 A→T 9.2% I52N (ATC→AAC)  CJ019_03932 ← hypothetical protein
JC 4,257,334 +CGCGGCCG 100% intergenic (‑280/+8) CJ019_03991 ← / ← CJ019_03994 putative ABC transporter ATP‑binding protein/hypothetical protein
RA 4,259,370:1 +A 15.6% coding (85/1254 nt) nicP_10 ← Porin‑like protein NicP
RA 4,327,353 A→G 6.7% F5L (TTC→CTC)  mbtH ← Protein MbtH
RA 4,624,111 C→A 14.9% intergenic (+117/+181) CJ019_04367 → / ← pgsA hypothetical protein/CDP‑diacylglycerol‑‑glycerol‑3‑phosphate 3‑phosphatidyltransferase
RA 4,627,056 C→T 100% G100D (GGC→GAC)  gacA ← Response regulator GacA
RA 4,654,515 A→C 32.7% T594P (ACC→CCC)  nuoG → NADH‑quinone oxidoreductase subunit G
RA 4,741,649 A→T 5.6% intergenic (+71/+1) CJ019_04477 → / ← mqsA Electron transfer flavoprotein‑ubiquinoneoxidoreductase/Antitoxin MqsA
RA 4,747,238 T→G 7.4% S294A (TCG→GCG)  macB → Macrolide export ATP‑binding/permease proteinMacB
RA 4,760,949 G→C 15.1% intergenic (+9/+46) ligM_KToptimized → / ← lip2 sequence generated by OPTIMIZER for KT2440/Lipase 2
RA 4,761,978 T→G 12.7% intergenic (‑27/+16) lip2 ← / ← grsB Lipase 2/Gramicidin S synthase 2
RA 4,765,167 T→G 7.9% T2414P (ACC→CCC)  grsB ← Gramicidin S synthase 2
RA 4,772,546 T→G 45.0% intergenic (‑140/+21) grsB ← / ← lgrB_1 Gramicidin S synthase 2/Linear gramicidin synthase subunit B
RA 4,788,943 T→G 8.6% S228R (AGC→CGC)  dat ← Diaminobutyrate‑‑2‑oxoglutarateaminotransferase
RA 4,797,995 A→C 14.2% G71G (GGT→GGG hcrC ← 4‑hydroxybenzoyl‑CoA reductase subunit gamma
RA 4,799,383 G→C 7.2% S550C (TCC→TGC)  CJ019_04509 ← hypothetical protein
RA 4,805,214 A→C 16.3% intergenic (+141/‑608) dsbG → / → CJ019_04513 Thiol:disulfide interchange protein DsbG/hypothetical protein
RA 4,805,234 A→T 18.1% intergenic (+161/‑588) dsbG → / → CJ019_04513 Thiol:disulfide interchange protein DsbG/hypothetical protein
RA 4,805,237 A→C 29.2% intergenic (+164/‑585) dsbG → / → CJ019_04513 Thiol:disulfide interchange protein DsbG/hypothetical protein
RA 4,805,499 C→A 6.7% intergenic (+426/‑323) dsbG → / → CJ019_04513 Thiol:disulfide interchange protein DsbG/hypothetical protein
RA 4,840,424 T→G 8.6% W620G (TGG→GGG)  copA_3 → putative copper‑importing P‑type ATPase A
RA 4,841,918 C→G 19.8% intergenic (+7/‑99) CJ019_04539 → / → hemN hypothetical protein/Oxygen‑independent coproporphyrinogen IIIoxidase
RA 4,841,928 G→C 30.3% intergenic (+17/‑89) CJ019_04539 → / → hemN hypothetical protein/Oxygen‑independent coproporphyrinogen IIIoxidase
RA 4,844,971 T→G 26.1% intergenic (+47/+72) apt → / ← recR Adenine phosphoribosyltransferase/Recombination protein RecR
RA 4,844,974 T→A 13.5% intergenic (+50/+69) apt → / ← recR Adenine phosphoribosyltransferase/Recombination protein RecR
RA 4,844,977 G→C 17.1% intergenic (+53/+66) apt → / ← recR Adenine phosphoribosyltransferase/Recombination protein RecR
RA 4,844,988 A→T 19.3% intergenic (+64/+55) apt → / ← recR Adenine phosphoribosyltransferase/Recombination protein RecR
RA 4,926,694 G→A 5.9% I357I (ATC→ATT srp54 ← Signal recognition particle 54 kDa protein
RA 4,927,190 T→G 32.2% Q192P (CAG→CCG)  srp54 ← Signal recognition particle 54 kDa protein
RA 5,096,415 C→G 25.2% intergenic (+28/‑162) CJ019_04792 → / → CJ019_04793 Putative pterin‑4‑alpha‑carbinolaminedehydratase/hypothetical protein
RA 5,154,737 C→T 5.3% D62D (GAC→GAT CJ019_04852 → hypothetical protein
RA 5,386,500 C→A 100% intergenic (‑509/+1142) CJ019_05070 ← / ← nptII hypothetical protein/neomycin phosphotransferase
RA 5,386,503 C→G 100% intergenic (‑512/+1139) CJ019_05070 ← / ← nptII hypothetical protein/neomycin phosphotransferase
RA 5,386,506 1 bp→GT 100% intergenic (‑515/+1136) CJ019_05070 ← / ← nptII hypothetical protein/neomycin phosphotransferase
RA 5,386,507:1 +C 100% intergenic (‑516/+1135) CJ019_05070 ← / ← nptII hypothetical protein/neomycin phosphotransferase
RA 5,386,510 2 bp→TA 100% intergenic (‑519/+1131) CJ019_05070 ← / ← nptII hypothetical protein/neomycin phosphotransferase
RA 5,386,512:1 +AA 100% intergenic (‑521/+1130) CJ019_05070 ← / ← nptII hypothetical protein/neomycin phosphotransferase
RA 5,386,514 T→C 100% intergenic (‑523/+1128) CJ019_05070 ← / ← nptII hypothetical protein/neomycin phosphotransferase
RA 5,386,517 G→C 100% intergenic (‑526/+1125) CJ019_05070 ← / ← nptII hypothetical protein/neomycin phosphotransferase
RA 5,386,519 G→A 100% intergenic (‑528/+1123) CJ019_05070 ← / ← nptII hypothetical protein/neomycin phosphotransferase
RA 5,386,811 C→T 100% intergenic (‑820/+831) CJ019_05070 ← / ← nptII hypothetical protein/neomycin phosphotransferase
RA 5,390,023 Δ1 bp 24.3% coding (117/1666 nt) ligH → 10‑formyltetrahydrofolate synthetase
JC 5,390,260 +GTGCCGATGGA 33.3% coding (354/1666 nt) ligH → 10‑formyltetrahydrofolate synthetase
RA 5,390,652 T→G 12.1% L249R (CTG→CGG)  ligH → 10‑formyltetrahydrofolate synthetase
RA 5,390,745 G→A 6.6% G280D (GGC→GAC)  ligH → 10‑formyltetrahydrofolate synthetase
RA 5,391,513 G→T 12.8% G536V (GGC→GTC)  ligH → 10‑formyltetrahydrofolate synthetase
JC 5,391,707 +54 bp 100% intergenic (+124/‑1253) ligH → / → CJ019_05072 10‑formyltetrahydrofolate synthetase/hypothetical protein
RA 5,408,980 T→C 8.0% K792R (AAG→AGG)  fhuA_3 ← Ferrichrome outer membrane transporter/phagereceptor
RA 5,569,213 A→C 5.5% C36G (TGC→GGC)  CJ019_05240 ← hypothetical protein
RA 5,612,860 C→T 5.8% A597T (GCC→ACC)  msbA ← Lipid A export ATP‑binding/permease proteinMsbA
RA 5,693,153 T→G 36.5% R228R (CGT→CGG pyrC' → Dihydroorotase‑like protein
RA 5,693,167 A→C 19.7% Q233P (CAG→CCG)  pyrC' → Dihydroorotase‑like protein
RA 5,693,172 A→C 19.4% T235P (ACC→CCC)  pyrC' → Dihydroorotase‑like protein
RA 5,693,175 A→C 43.8% S236R (AGC→CGC)  pyrC' → Dihydroorotase‑like protein
RA 5,693,183 T→G 33.8% R238R (CGT→CGG pyrC' → Dihydroorotase‑like protein
RA 5,760,593 Δ1 bp 100% coding (776/834 nt) CJ019_05400 → hypothetical protein
RA 5,861,107 G→A 5.6% R746R (CGC→CGT cadA_2 ← Cadmium‑transporting ATPase
RA 5,894,256 C→G 5.8% L102L (CTG→CTC sbp_2 ← Sulfate‑binding protein
RA 5,950,136 Δ1 bp 6.4% coding (75/2244 nt) ppk ← Polyphosphate kinase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ ACB143 87998 88052 55 14 [12] [13] 15 [CJ019_00094]–[CJ019_00095] [CJ019_00094],[CJ019_00095]
* * ÷ ACB143 517195 517295 101 17 [11] [13] 14 tyrS/birA Tyrosine‑‑tRNA ligase/Bifunctional ligase/repressor BirA
* * ÷ ACB143 674269 674380 112 15 [11] [13] 19 [nimR_1]–[mcpH_2] [nimR_1],[mcpH_2]
* * ÷ ACB143 1396584 1396674 91 14 [11] [13] 14 [CJ019_01313]–[mcpU_1] [CJ019_01313],[mcpU_1]
* * ÷ ACB143 1499940 1500088 149 14 [13] [13] 17 [sspA] [sspA]
* * ÷ ACB143 1914570 1914663 94 15 [13] [13] 14 [ppsC_1]–[CJ019_01830] [ppsC_1],[CJ019_01830]
* * ÷ ACB143 1923391 1923526 136 14 [13] [13] 14 [apeB] [apeB]
* * ÷ ACB143 2467190 2467208 19 23 [1] [9] 23 CJ019_02325/tal hypothetical protein/Transaldolase
* * ÷ ACB143 3046188 3046335 148 14 [12] [13] 16 [rcsC_5]–[degU_2] [rcsC_5],[degU_2]
* * ÷ ACB143 3769380 3769481 102 14 [13] [13] 14 CJ019_03581/CJ019_03582 hypothetical protein/hypothetical protein
* * ÷ ACB143 3944691 3944776 86 14 [13] [10] 14 CJ019_03729/CJ019_03730 hypothetical protein/hypothetical protein
* * ÷ ACB143 4163765 4163853 89 14 [13] [13] 14 katG/leuO_2 Catalase‑peroxidase/HTH‑type transcriptional regulator LeuO
* * ÷ ACB143 4344755 4344829 75 15 [13] [13] 14 nmoA Nitronate monooxygenase
* * ÷ ACB143 4521552 4521687 136 15 [13] [13] 14 [CJ019_04289] [CJ019_04289]
* * ÷ ACB143 4732383 4732479 97 14 [13] [13] 15 [CJ019_04468] [CJ019_04468]
* * ÷ ACB143 4944234 4944335 102 14 [12] [13] 14 [CJ019_04642] [CJ019_04642]
* * ÷ ACB143 5250136 5250222 87 14 [13] [12] 16 [CJ019_04947]–[CJ019_04948] [CJ019_04947],[CJ019_04948]
* * ÷ ACB143 5737631 5737694 64 14 [13] [10] 14 CJ019_05378/blc_2 8‑oxoguanine deaminase/Outer membrane lipoprotein Blc
* * ÷ ACB143 5900321 5900394 74 14 [12] [12] 14 [CJ019_05521] [CJ019_05521]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? ACB143 = 522939NA (NA)3 (0.070)
+35 bp
3/170 NT 6.7% intergenic (+5756/‑198) tyrS/birA Tyrosine‑‑tRNA ligase/Bifunctional ligase/repressor BirA
?ACB143 525641 = 59 (1.000)intergenic (+39/‑145) PP_RS08755/tufA_1 tRNA‑Asp/Elongation factor Tu‑A
* ? ACB143 1237160 =35 (0.590)13 (0.220) 11/236 NT 18.6% coding (378/675 nt) rnt Ribonuclease T
?ACB143 2170757 = 79 (1.360)intergenic (‑772/‑65) arsR1/CJ019_02060 Arsenic resistance transcriptional regulatorArsR1/IS1182 family transposase ISPpu16
* ? ACB143 = 123716135 (0.590)9 (0.150) 9/236 NT 10.9% coding (377/675 nt) rnt Ribonuclease T
?ACB143 = 2172433 113 (1.940)intergenic (+175/+93) CJ019_02060/CJ019_02061 IS1182 family transposase ISPpu16/hypothetical protein
* ? ACB143 3359593 =26 (0.440)230 (3.890) 86/240 NT 86.3% intergenic (+219/‑151) pikAII_1/CJ019_03160 Narbonolide/10‑deoxymethynolide synthase PikA2,modules 3 and 4/Tn3 family transposase ISPa42
?ACB143 4740816 = 47 (0.790)coding (903/1665 nt) CJ019_04477 Electron transfer flavoprotein‑ubiquinoneoxidoreductase
* ? ACB143 = 337660552 (0.880)293 (4.950) 81/240 NT 86.6% coding (501/546 nt) CJ019_03184 hypothetical protein
?ACB143 = 4850748 39 (0.660)coding (435/489 nt) CJ019_04550 hypothetical protein
* ? ACB143 = 415620235 (0.590)7 (0.130) 7/226 NT 19.2% coding (873/1314 nt) pssA CDP‑diacylglycerol‑‑serineO‑phosphatidyltransfer a se
?ACB143 4260095 = 26 (0.470)coding (803/1341 nt) pcaK_3 4‑hydroxybenzoate transporter PcaK
* ? ACB143 = 419557834 (0.570)5 (0.090) 5/234 NT 10.4% coding (5826/6624 nt) CJ019_03944 hypothetical protein
?ACB143 4300622 = 53 (0.920)coding (440/1425 nt) CJ019_04036 hypothetical protein
* ? ACB143 5542506 =NA (NA)5 (0.090) 5/236 NT 8.6% intergenic (‑13/+416) CJ019_03622/rlmB IS110 family transposase ISPpu9/23S rRNA (guanosine‑2'‑O‑)‑methyltransferaseRlmB
?ACB143 = 5618433 54 (0.910)coding (19/774 nt) CJ019_05279 hypothetical protein
* ? ACB143 = 5542851NA (NA)12 (0.210) 11/234 NT 18.6% intergenic (‑358/+71) CJ019_03622/rlmB IS110 family transposase ISPpu9/23S rRNA (guanosine‑2'‑O‑)‑methyltransferaseRlmB
?ACB143 5618432 = 54 (0.910)coding (20/774 nt) CJ019_05279 hypothetical protein
* ? ACB143 = 598595122 (0.370)3 (0.050) 3/224 NT 11.2% intergenic (+51/‑638) kefC_3/CJ019_05604 Glutathione‑regulated potassium‑efflux systemprotein KefC/hypothetical protein
?ACB143 5986173 = 27 (0.490)intergenic (+273/‑416) kefC_3/CJ019_05604 Glutathione‑regulated potassium‑efflux systemprotein KefC/hypothetical protein