breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 44,293 Δ1 bp 100% intergenic (+124/‑367) oprD_1 → / → czcR_2 Porin D/Transcriptional activator protein CzcR
RA 96,466 T→G 27.5% intergenic (+107/‑86) cynT → / → CJ019_00104 Carbonic anhydrase 1/hypothetical protein
RA 96,480 T→G 33.4% intergenic (+121/‑72) cynT → / → CJ019_00104 Carbonic anhydrase 1/hypothetical protein
RA 120,975 Δ1 bp 100% intergenic (+272/+100) polA → / ← engB DNA polymerase I/putative GTP‑binding protein EngB
RA 179,484 T→C 100% C316R (TGC→CGC)  CJ019_00182 → hypothetical protein
RA 356,383 G→A 12.7% V50V (GTC→GTT lcdH_2 ← L‑carnitine dehydrogenase
RA 382,476 A→C 43.9% E11A (GAG→GCG)  soxD → Sarcosine oxidase subunit delta
RA 515,063 G→A 45.2% D236D (GAC→GAT mepM ← Murein DD‑endopeptidase MepM
RA 541,976 C→T 8.6% D592D (GAC→GAT fusA → Elongation factor G 1
RA 601,357 C→T 10.5% P60S (CCT→TCT)  btuF → Vitamin B12‑binding protein
RA 620,951 G→A 7.8% intergenic (‑33/+75) CJ019_00589 ← / ← eutC hypothetical protein/Ethanolamine ammonia‑lyase light chain
RA 1,198,856 A→T 10.0% intergenic (‑42/+48) CJ019_01129 ← / ← CJ019_01130 hypothetical protein/hypothetical protein
RA 1,327,427 C→T 9.5% R234W (CGG→TGG)  CJ019_01244 → hypothetical protein
RA 1,505,955 A→G 8.3% intergenic (+271/‑481) rsmI → / → mraZ Ribosomal RNA small subunit methyltransferase I/Transcriptional regulator MraZ
RA 1,589,731 G→T 8.4% G112G (GGC→GGA dctD_3 ← C4‑dicarboxylate transport transcriptionalregulatory protein DctD
RA 1,637,682 T→C 8.5% K39R (AAG→AGG)  lon_1 ← Lon protease
RA 1,670,685 A→C 36.8% intergenic (+22/‑109) thrC_2 → / → CJ019_01569 Threonine synthase/hypothetical protein
RA 1,677,216 G→A 7.4% V247I (GTC→ATC)  namA_2 → NADPH dehydrogenase
RA 1,718,463 T→G 32.0% Q65P (CAG→CCG)  CJ019_01614 ← hypothetical protein
RA 1,791,678 T→G 34.3% L182R (CTG→CGG)  lpxB → Lipid‑A‑disaccharide synthase
RA 1,830,632 G→A 7.3% P65P (CCG→CCA CJ019_01746 → hypothetical protein
RA 1,834,908 T→G 37.1% T38P (ACC→CCC)  ldhA ← D‑lactate dehydrogenase
RA 1,839,275 G→T 12.9% L170L (CTG→CTT pfeS_2 → Sensor protein PfeS
RA 2,018,148 C→A 9.6% R31S (CGT→AGT)  mshA_4 → D‑inositol‑3‑phosphate glycosyltransferase
RA 2,076,973 G→A 8.7% L190L (CTG→CTA CJ019_01978 → hypothetical protein
RA 2,084,843 C→T 7.1% A371V (GCG→GTG)  deaD → ATP‑dependent RNA helicase DeaD
RA 2,094,817 T→G 66.5% Q183P (CAG→CCG)  CJ019_01995 ← hypothetical protein
RA 2,231,813 A→C 36.6% G291G (GGT→GGG emrA_2 ← Colistin resistance protein EmrA
RA 2,491,272 A→C 44.4% H33P (CAC→CCC)  iutA → Ferric aerobactin receptor
RA 2,605,842 G→A 10.2% G575D (GGC→GAC)  CJ019_02463 → hypothetical protein
RA 2,642,546 G→A 11.8% A136T (GCC→ACC)  ynhG → putative L,D‑transpeptidase YnhG
RA 3,021,386 C→A 7.9% intergenic (+203/‑46) hisC_6 → / → CJ019_02839 Histidinol‑phosphate aminotransferase/hypothetical protein
RA 3,024,446 C→T 10.5% Y216Y (TAC→TAT mgtB → Magnesium‑transporting ATPase, P‑type 1
RA 3,061,793 G→A 100% S592N (AGC→AAC)  qedA_2 → Quinoprotein alcohol dehydrogenase (cytochromec)
RA 3,072,630 T→G 28.3% intergenic (‑308/+126) CJ019_02884 ← / ← yabJ_2 putative protein/2‑iminobutanoate/2‑iminopropanoate deaminase
RA 3,134,642 G→T 12.1% V168F (GTC→TTC)  CJ019_02952 → hypothetical protein
RA 3,157,749 C→A 9.8% P68H (CCT→CAT)  CJ019_02972 → hypothetical protein
RA 3,267,810 T→G 28.8% L171V (TTG→GTG)  eamA → putative amino‑acid metabolite efflux pump
RA 3,362,995 T→A 5.8% L74Q (CTG→CAG)  CJ019_03161 → hypothetical protein
RA 3,440,343 A→C 100% S18R (AGC→CGC)  CJ019_03266 → hypothetical protein
RA 3,440,348 T→G 53.5% A19A (GCT→GCG CJ019_03266 → hypothetical protein
RA 3,667,362 T→C 8.9% H84R (CAT→CGT)  pyrR_1 ← Bifunctional protein PyrR
RA 3,761,329 A→C 30.5% E96A (GAG→GCG)  CJ019_03574 → hypothetical protein
RA 3,945,826 C→A 8.4% K123N (AAG→AAT CJ019_03731 ← hypothetical protein
RA 3,957,060 A→T 11.1% Y197N (TAC→AAC)  dmlR_13 ← HTH‑type transcriptional regulator DmlR
RA 3,957,251 G→A 45.5% P133L (CCG→CTG)  dmlR_13 ← HTH‑type transcriptional regulator DmlR
RA 3,957,351 G→A 47.5% H100Y (CAC→TAC)  dmlR_13 ← HTH‑type transcriptional regulator DmlR
RA 3,970,221 C→T 7.8% D98N (GAC→AAC)  CJ019_03750 ← putative protein
RA 3,986,622 T→G 35.0% D31A (GAT→GCT)  CJ019_03763 ← hypothetical protein
RA 3,989,732 T→C 6.1% L31P (CTG→CCG)  CJ019_03768 → hypothetical protein
RA 4,077,164 T→G 37.7% L173R (CTG→CGG)  artJ_2 → ABC transporter arginine‑binding protein 1
RA 4,092,063 A→C 21.5% V91G (GTC→GGC)  CJ019_03853 ← 2‑haloacrylate reductase
RA 4,096,610 G→A 7.5% A94V (GCC→GTC)  CJ019_03858 ← hypothetical protein
RA 4,135,592 A→C 17.0% N27T (AAC→ACC)  tgnC → (Z)‑2‑((N‑methylformamido)methylene)‑5‑hydroxybu tyrolactone dehydrogenase
RA 4,135,607 A→C 47.4% Q32P (CAG→CCG)  tgnC → (Z)‑2‑((N‑methylformamido)methylene)‑5‑hydroxybu tyrolactone dehydrogenase
RA 4,145,586 A→C 24.1% intergenic (+131/+20) CJ019_03903 → / ← nicP_9 hypothetical protein/Porin‑like protein NicP
RA 4,229,020 C→G 6.2% D116H (GAC→CAC)  CJ019_03968 ← hypothetical protein
RA 4,245,154 T→G 49.8% D175A (GAT→GCT)  CJ019_03982 ← Crotonyl‑CoA hydratase
RA 4,258,563 A→C 24.4% D44A (GAC→GCC)  pht2 → Phthalate 4,5‑dioxygenase oxygenase reductasesubunit
JC 4,263,135 Δ15 bp 6.8% intergenic (‑190/‑357) pcaK_3 ← / → mrdA_1 4‑hydroxybenzoate transporter PcaK/Peptidoglycan D,D‑transpeptidase MrdA
RA 4,291,840 T→G 27.9% intergenic (‑142/‑20) sdhL ← / → CJ019_04025 Shikimate dehydrogenase‑like protein/hypothetical protein
RA 4,434,267 A→C 31.3% intergenic (+590/‑259) dmlR_17 → / → CJ019_04207 HTH‑type transcriptional regulator DmlR/hypothetical protein
RA 4,440,130 C→A 6.7% Q360H (CAG→CAT nicC ← 6‑hydroxynicotinate 3‑monooxygenase
RA 4,505,789 G→T 8.1% P94Q (CCG→CAG)  lolA ← Outer‑membrane lipoprotein carrier protein
RA 4,666,745 T→G 34.6% intergenic (‑90/‑131) lasR ← / → btuB_4 Transcriptional activator protein LasR/Vitamin B12 transporter BtuB
RA 4,670,287 C→T 7.5% R645Q (CGG→CAG)  adiA ← Biodegradative arginine decarboxylase
RA 4,749,286 T→G 26.8% S294A (TCG→GCG)  macB → Macrolide export ATP‑binding/permease proteinMacB
RA 4,789,443 A→C 31.1% V230G (GTG→GGG)  dat ← Diaminobutyrate‑‑2‑oxoglutarateaminotransferase
RA 4,825,420 T→A 7.9% C35S (TGC→AGC)  CJ019_04521 → hypothetical protein
RA 4,874,567 A→C 35.8% intergenic (+13/‑174) CJ019_04568 → / → uacT_1 hypothetical protein/Uric acid transporter UacT
RA 4,970,678 G→A 8.5% P346L (CCC→CTC)  flgI ← Flagellar P‑ring protein
RA 4,986,317 A→T 9.1% I150F (ATC→TTC)  bkdA1 → 2‑oxoisovalerate dehydrogenase subunit alpha
RA 5,023,365 T→C 72.2% S40P (TCT→CCT)  proP_6 → Proline/betaine transporter
RA 5,055,964 Δ1 bp 7.3% coding (479/762 nt) CJ019_04749 → IS630 family transposase ISPpu1
RA 5,284,713 T→G 25.5% F162C (TTT→TGT)  cmpR_6 → HTH‑type transcriptional activator CmpR
RA 5,336,288 G→A 7.0% D258N (GAT→AAT)  panC → Pantothenate synthetase
RA 5,624,908 C→T 12.8% F203F (TTC→TTT putA → Bifunctional protein PutA
RA 5,624,911 C→T 12.0% V204V (GTC→GTT putA → Bifunctional protein PutA
RA 5,624,914 C→T 12.2% N205N (AAC→AAT putA → Bifunctional protein PutA
RA 5,689,816 A→C 51.5% S236R (AGC→CGC)  pyrC' → Dihydroorotase‑like protein
RA 5,757,234 Δ1 bp 100% coding (776/834 nt) CJ019_05400 → hypothetical protein
RA 5,818,139 C→A 8.9% L19M (CTG→ATG)  CJ019_05447 → hypothetical protein
JC 5,924,085 Δ6 bp 5.8% coding (133‑138/1224 nt) ubiI ← 2‑octaprenylphenol hydroxylase
RA 5,982,960 A→T 11.8% intergenic (+419/‑270) kefC_3 → / → CJ019_05604 Glutathione‑regulated potassium‑efflux systemprotein KefC/hypothetical protein
RA 6,036,532 C→A 8.9% Q183K (CAG→AAG)  CJ019_05653 → hypothetical protein
RA 6,097,340 C→A 7.6% A113D (GCC→GAC)  CJ019_05710 → hypothetical protein
RA 6,125,178 T→C 5.5% Q60R (CAG→CGG)  copB_2 ← Copper resistance protein B
RA 6,172,167 A→T 8.9% F249Y (TTC→TAC)  atpA ← ATP synthase subunit alpha

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ KT2440 87981 88060 80 12 [10] [10] 13 [CJ019_00094]–[CJ019_00095] [CJ019_00094],[CJ019_00095]
* * ÷ KT2440 161242 161381–161316 75–140 12 [11] [11] 12 [uctC_1] [uctC_1]
* * ÷ KT2440 277592 277677 86 12 [11] [11] 12 [betT2]–[tauD_2] [betT2],[tauD_2]
* * ÷ KT2440 329220 329368 149 17 [10] [6] 13 [CJ019_00302] [CJ019_00302]
* * ÷ KT2440 517190 517387 198 17 [10] [11] 12 tyrS/birA Tyrosine‑‑tRNA ligase/Bifunctional ligase/repressor BirA
* * ÷ KT2440 607275 607400 126 13 [10] [7] 12 dxs/ispA 1‑deoxy‑D‑xylulose‑5‑phosphate synthase/Farnesyl diphosphate synthase
* * ÷ KT2440 641268 641354 87 12 [11] [11] 12 [acoR_2] [acoR_2]
* * ÷ KT2440 674269 674380 112 13 [11] [8] 14 [nimR_1]–[mcpH_2] [nimR_1],[mcpH_2]
* * ÷ KT2440 789844 789951 108 12 [11] [11] 12 [CJ019_00751] [CJ019_00751]
* * ÷ KT2440 856905 856997 93 13 [10] [11] 12 [hemH]–[uraA] [hemH],[uraA]
* * ÷ KT2440 876815 876981 167 12 [11] [10] 12 CJ019_00843/malT putative protein/HTH‑type transcriptional regulator MalT
* * ÷ KT2440 1000522 1000679 158 14 [11] [11] 13 [fecA_1]–[osmV] [fecA_1],[osmV]
* * ÷ KT2440 1106836 1106965 130 12 [11] [11] 12 [hupB_1]–[rlmF] [hupB_1],[rlmF]
* * ÷ KT2440 1107881 1108001 121 12 [11] [11] 12 rlmF/valS Ribosomal RNA large subunit methyltransferase F/Valine‑‑tRNA ligase
* * ÷ KT2440 1119629 1119715 87 13 [11] [9] 17 tdcG_2/gcvP_1 L‑serine dehydratase TdcG/Glycine dehydrogenase (decarboxylating)
* * ÷ KT2440 1149276 1149475 200 12 [11] [10] 12 [sasA_4] [sasA_4]
* * ÷ KT2440 1304745 1304852 108 13 [11] [10] 14 CJ019_01223/rapA putative signaling protein/RNA polymerase‑associated protein RapA
* * ÷ KT2440 1360587 1360999 413 13 [11] [9] 12 [CJ019_01275] [CJ019_01275]
* * ÷ KT2440 1376290 1376321 32 12 [10] [7] 14 CJ019_01289 hypothetical protein
* * ÷ KT2440 1396593 1396679 87 13 [9] [9] 13 [mcpU_1] [mcpU_1]
* * ÷ KT2440 1411275 1411322 48 12 [11] [10] 12 [purC] [purC]
* * ÷ KT2440 1457213 1457299 87 12 [9] [11] 12 [algL]–[algX] [algL],[algX]
* * ÷ KT2440 1492461 1492581 121 12 [6] [11] 14 [CJ019_01406] [CJ019_01406]
* * ÷ KT2440 1499958 1500063 106 13 [11] [11] 12 [sspA] [sspA]
* * ÷ KT2440 1696279 1696332 54 13 [11] [11] 12 lysS/CJ019_01592 Lysine‑‑tRNA ligase/hypothetical protein
* * ÷ KT2440 1697651 1697722 72 12 [10] [11] 13 [CJ019_01593] [CJ019_01593]
* * ÷ KT2440 1705094 1705206 113 12 [11] [10] 13 ppc/adk Phosphoenolpyruvate carboxylase/Adenylate kinase
* * ÷ KT2440 1833103 1833171 69 12 [11] [9] 12 [resA_1]–[CJ019_01751] [resA_1],[CJ019_01751]
* * ÷ KT2440 1835848 1835908 61 12 [10] [8] 13 barA Signal transduction histidine‑protein kinaseBarA
* * ÷ KT2440 1914571 1914655 85 15 [9] [11] 12 [ppsC_1]–[CJ019_01830] [ppsC_1],[CJ019_01830]
* * ÷ KT2440 1923425 1923524 100 12 [10] [11] 12 [apeB] [apeB]
* * ÷ KT2440 1940792 1940944 153 12 [11] [11] 15 [CJ019_01857] [CJ019_01857]
* * ÷ KT2440 2043583 2043728 146 14 [7] [10] 13 smrA/CJ019_01939 putative DNA endonuclease SmrA/hypothetical protein
* * ÷ KT2440 2288815 2288934 120 14 [10] [9] 13 CJ019_02171/CJ019_02172 hypothetical protein/hypothetical protein
* * ÷ KT2440 3046182 3046308 127 12 [11] [11] 12 [rcsC_5]–[degU_2] [rcsC_5],[degU_2]
* * ÷ KT2440 3069114 3069170 57 12 [10] [11] 12 CJ019_02881/CJ019_02882 hypothetical protein/putative protein
* * ÷ KT2440 3280251 3280364 114 12 [11] [11] 12 [CJ019_03085]–[xthA_2] [CJ019_03085],[xthA_2]
* * ÷ KT2440 3459854 3459950 97 12 [10] [11] 13 CJ019_03284 hypothetical protein
* * ÷ KT2440 4163802 4163866 65 13 [11] [11] 12 katG/leuO_2 Catalase‑peroxidase/HTH‑type transcriptional regulator LeuO
* * ÷ KT2440 4332112 4332156 45 13 [11] [8] 13 aes/CJ019_04068 Acetyl esterase/hypothetical protein
* * ÷ KT2440 4333672 4333769 98 14 [11] [8] 12 ribBA_2/potD_2 Riboflavin biosynthesis protein RibBA/Spermidine/putrescine‑binding periplasmicprotein
* * ÷ KT2440 4337914 4337975 62 12 [11] [9] 12 [btuD_7] [btuD_7]
* * ÷ KT2440 4346805 4346849 45 17 [9] [10] 13 nmoA Nitronate monooxygenase
* * ÷ KT2440 4497380 4497487 108 12 [11] [11] 14 [xanP]–[dsrE] [xanP],[dsrE]
* * ÷ KT2440 4523610 4523710 101 12 [11] [10] 15 [CJ019_04289] [CJ019_04289]
* * ÷ KT2440 4734457 4734521 65 13 [10] [11] 12 gltA/CJ019_04468 Citrate synthase/hypothetical protein
* * ÷ KT2440 4883384 4883520 137 12 [11] [10] 12 hyi/glxR Hydroxypyruvate isomerase/2‑hydroxy‑3‑oxopropionate reductase
* * ÷ KT2440 4944706 4944847 142 13 [11] [11] 12 [CJ019_04642] [CJ019_04642]
* * ÷ KT2440 5167045 5167090 46 12 [11] [6] 16 fadD_1/fadD_2 Long‑chain‑fatty‑acid‑‑CoA ligase/Long‑chain‑fatty‑acid‑‑CoA ligase
* * ÷ KT2440 5188666 5188756 91 12 [10] [11] 12 [CJ019_04882]–[pxpC_2] [CJ019_04882],[pxpC_2]
* * ÷ KT2440 5193419 5193530 112 12 [11] [11] 13 [gltR_4]–[CJ019_04888] [gltR_4],[CJ019_04888]
* * ÷ KT2440 5250609 5250721 113 12 [9] [10] 12 [CJ019_04947]–[CJ019_04948] [CJ019_04947],[CJ019_04948]
* * ÷ KT2440 5433061 5433186 126 13 [11] [11] 12 [rluE] [rluE]
* * ÷ KT2440 5478914 5479038 125 12 [11] [11] 12 [purH] [purH]
* * ÷ KT2440 5500300 5500503 204 12 [8] [11] 12 [CJ019_05176] [CJ019_05176]
* * ÷ KT2440 5646783 5646858 76 20 [9] [11] 12 CJ019_05302/CJ019_05303 hypothetical protein/hypothetical protein
* * ÷ KT2440 5675179 5675302 124 12 [10] [11] 12 CJ019_05328/CJ019_05329 hypothetical protein/hypothetical protein
* * ÷ KT2440 5810709 5810971 263 12 [10] [11] 12 [pilT] [pilT]
* * ÷ KT2440 5896961 5897040 80 12 [10] [11] 13 [CJ019_05521] [CJ019_05521]
* * ÷ KT2440 5937468 5937492 25 13 [11] [6] 12 qorA_2/chaC Quinone oxidoreductase 1/Glutathione‑specificgamma‑glutamylcyclotransferas e

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? KT2440 4201049 =59 (1.390)4 (0.090) 4/234 NT 6.1% coding (372/6624 nt) CJ019_03944 hypothetical protein
?KT2440 = 4292766 65 (1.530)coding (130/315 nt) CJ019_04027 hypothetical protein
* ? KT2440 4918800 =0 (0.000)45 (1.090) 33/228 NT 100% coding (7/789 nt) soj_1 Sporulation initiation inhibitor protein Soj
?KT2440 = 5539492 NA (NA)intergenic (‑358/+71) CJ019_03622/rlmB IS110 family transposase ISPpu9/23S rRNA (guanosine‑2'‑O‑)‑methyltransferaseRlmB
* ? KT2440 = 49188010 (0.000)35 (0.840) 26/230 NT 100% coding (6/789 nt) soj_1 Sporulation initiation inhibitor protein Soj
?KT2440 5216408 = NA (NA)intergenic (‑50/+386) rhaS_5/CJ019_01212 HTH‑type transcriptional activator RhaS/IS110 family transposase ISPpu9