breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 12,483 G→A 9.4% T618T (ACG→ACA CJ019_00019 → hypothetical protein
RA 44,293 Δ1 bp 100% intergenic (+124/‑367) oprD_1 → / → czcR_2 Porin D/Transcriptional activator protein CzcR
RA 86,554 G→A 6.9% Y188Y (TAC→TAT limB ← Limonene 1,2‑monooxygenase
RA 120,975 Δ1 bp 100% intergenic (+272/+100) polA → / ← engB DNA polymerase I/putative GTP‑binding protein EngB
RA 150,743 T→G 55.1% E27A (GAG→GCG)  CJ019_00156 ← hypothetical protein
RA 169,760 A→C 11.4% intergenic (+724/+2963) PP_RS08750 → / ← fhuA_1 tRNA‑Val/Ferrichrome outer membrane transporter/phagereceptor
RA 169,792 G→A 8.9% intergenic (+756/+2931) PP_RS08750 → / ← fhuA_1 tRNA‑Val/Ferrichrome outer membrane transporter/phagereceptor
RA 169,811 C→A 10.5% intergenic (+775/+2912) PP_RS08750 → / ← fhuA_1 tRNA‑Val/Ferrichrome outer membrane transporter/phagereceptor
RA 169,812 T→C 7.5% intergenic (+776/+2911) PP_RS08750 → / ← fhuA_1 tRNA‑Val/Ferrichrome outer membrane transporter/phagereceptor
RA 169,813 A→G 10.4% intergenic (+777/+2910) PP_RS08750 → / ← fhuA_1 tRNA‑Val/Ferrichrome outer membrane transporter/phagereceptor
RA 169,817 T→A 10.4% intergenic (+781/+2906) PP_RS08750 → / ← fhuA_1 tRNA‑Val/Ferrichrome outer membrane transporter/phagereceptor
RA 169,819 T→G 11.5% intergenic (+783/+2904) PP_RS08750 → / ← fhuA_1 tRNA‑Val/Ferrichrome outer membrane transporter/phagereceptor
RA 169,820 T→C 10.6% intergenic (+784/+2903) PP_RS08750 → / ← fhuA_1 tRNA‑Val/Ferrichrome outer membrane transporter/phagereceptor
RA 169,821 A→G 11.4% intergenic (+785/+2902) PP_RS08750 → / ← fhuA_1 tRNA‑Val/Ferrichrome outer membrane transporter/phagereceptor
RA 169,822 G→T 10.0% intergenic (+786/+2901) PP_RS08750 → / ← fhuA_1 tRNA‑Val/Ferrichrome outer membrane transporter/phagereceptor
RA 179,484 T→C 100% C316R (TGC→CGC)  CJ019_00182 → hypothetical protein
RA 252,598 G→A 6.4% G362D (GGC→GAC)  CJ019_00227 → hypothetical protein
RA 315,782 T→A 12.6% Q503L (CAG→CTG)  cntO_1 ← Metal‑pseudopaline receptor CntO
RA 383,395 G→A 8.5% G207S (GGC→AGC)  soxA_1 → Sarcosine oxidase subunit alpha
RA 573,463 C→T 7.6% C258C (TGC→TGT fdhE → Protein FdhE
RA 635,862 G→A 8.3% R59C (CGC→TGC)  acoC ← Dihydrolipoyllysine‑residue acetyltransferasecomponent of acetoin cleaving system
RA 656,905 A→C 25.6% T241P (ACG→CCG)  yfhM → Alpha‑2‑macroglobulin
RA 759,412 C→G 45.7% intergenic (+21/‑147) mmuM_1 → / → CJ019_00729 Homocysteine S‑methyltransferase/L‑methionine gamma‑lyase
RA 759,419 A→C 47.7% intergenic (+28/‑140) mmuM_1 → / → CJ019_00729 Homocysteine S‑methyltransferase/L‑methionine gamma‑lyase
RA 1,063,986 T→G 27.2% D151A (GAC→GCC)  CJ019_01001 ← hypothetical protein
RA 1,118,296 T→G 10.7% T435P (ACC→CCC)  tdcG_2 ← L‑serine dehydratase TdcG
RA 1,138,494 T→G 46.6% intergenic (‑108/+33) pigA_1 ← / ← hemR Heme oxygenase PigA/Hemin receptor
RA 1,342,117 A→G 6.2% intergenic (‑530/+224) nicP_1 ← / ← CJ019_01258 Porin‑like protein NicP/hypothetical protein
RA 1,362,136 C→T 9.0% R241W (CGG→TGG)  CJ019_01276 → hypothetical protein
RA 1,422,128 G→C 12.7% W410S (TGG→TCG)  mqo_2 → Malate:quinone oxidoreductase
RA 1,625,302 T→C 8.3% S334P (TCC→CCC)  lepA → Elongation factor 4
RA 1,670,685 A→C 41.8% intergenic (+22/‑109) thrC_2 → / → CJ019_01569 Threonine synthase/hypothetical protein
RA 1,670,696 A→C 44.2% intergenic (+33/‑98) thrC_2 → / → CJ019_01569 Threonine synthase/hypothetical protein
RA 1,720,951 T→G 39.0% D90A (GAC→GCC)  plsB ← Glycerol‑3‑phosphate acyltransferase
RA 1,720,958 T→G 37.4% T88P (ACC→CCC)  plsB ← Glycerol‑3‑phosphate acyltransferase
RA 1,720,970 A→C 32.1% F84V (TTC→GTC)  plsB ← Glycerol‑3‑phosphate acyltransferase
RA 1,721,870 A→C 36.4% V98G (GTC→GGC)  CJ019_01617 ← hypothetical protein
RA 1,807,474 G→A 100% G258D (GGC→GAC)  yeiG → S‑formylglutathione hydrolase YeiG
RA 1,869,832 G→A 6.3% L197L (TTG→TTA CJ019_01787 → hypothetical protein
RA 1,900,316 A→C 26.6% V160G (GTC→GGC)  CJ019_01816 ← 2‑ketogluconate reductase
RA 1,923,103 C→T 7.1% L15L (TTG→TTA CJ019_01837 ← hypothetical protein
RA 2,031,520 G→T 100% T339T (ACC→ACA wecB ← UDP‑N‑acetylglucosamine 2‑epimerase
RA 2,219,961 A→T 8.3% F166L (TTT→TTA CJ019_02103 ← hypothetical protein
RA 2,246,497 G→A 6.3% S193N (AGC→AAC)  COQ5 → 2‑methoxy‑6‑polyprenyl‑1,4‑benzoquinolmethylase, mitochondrial
RA 2,264,985 T→C 11.6% intergenic (+136/‑6) PP_RS08755 → / → PP_RS08755 tRNA‑Asp/tRNA‑Asp
RA 2,266,996 T→G 22.9% N143T (AAC→ACC)  CJ019_02153 ← putative HTH‑type transcriptional regulator
RA 2,508,222 G→A 5.9% R74R (CGC→CGT phnX_2 ← Phosphonoacetaldehyde hydrolase
RA 2,662,000 T→A 8.5% H265Q (CAT→CAA mmgE → Citrate/2‑methylcitrate dehydratase
RA 2,744,444 A→C 33.6% N64H (AAC→CAC)  czcC_2 → Cobalt‑zinc‑cadmium resistance protein CzcC
RA 2,839,289 C→T 100% intergenic (‑117/‑88) CJ019_02692 ← / → CJ019_02693 hypothetical protein/hypothetical protein
RA 2,858,207 A→G 19.9% F78S (TTC→TCC)  nicP_5 ← Porin‑like protein NicP
RA 2,869,602 T→C 10.6% K277R (AAG→AGG)  CJ019_02729 ← hypothetical protein
RA 2,910,766 A→C 20.6% K2243N (AAA→AAC CJ019_02759 → hypothetical protein
RA 3,002,898 G→A 9.7% L70L (CTG→CTA bcsE → Cyclic di‑GMP binding protein BcsE
RA 3,061,793 G→A 100% S592N (AGC→AAC)  qedA_2 → Quinoprotein alcohol dehydrogenase (cytochromec)
RA 3,179,891 C→T 9.2% A150A (GCC→GCT actP_2 → Cation/acetate symporter ActP
RA 3,210,887 A→C 41.5% Q206P (CAG→CCG)  oprJ_2 → Outer membrane protein OprJ
RA 3,231,966 T→G 19.0% F14V (TTC→GTC)  lgoT_2 → putative L‑galactonate transporter
RA 3,285,727 A→C 52.4% T652P (ACT→CCT)  tamB → Translocation and assembly module subunit TamB
RA 3,308,725 A→C 33.7% L164R (CTG→CGG)  CJ019_03110 ← hypothetical protein
RA 3,449,038 G→C 9.3% intergenic (‑91/+2) acsA_3 ← / ← eco Acetyl‑coenzyme A synthetase/Ecotin
RA 3,491,319 A→C 43.8% intergenic (‑681/+40) CJ019_03306 ← / ← CJ019_03307 hypothetical protein/hypothetical protein
RA 3,491,323 T→G 61.9% intergenic (‑685/+36) CJ019_03306 ← / ← CJ019_03307 hypothetical protein/hypothetical protein
RA 3,653,349 A→C 19.7% V148G (GTG→GGG)  mtnB_2 ← Methylthioribulose‑1‑phosphate dehydratase
RA 3,761,329 A→C 44.3% E96A (GAG→GCG)  CJ019_03574 → hypothetical protein
RA 3,850,110 A→C 16.3% F117V (TTC→GTC)  eamB_1 ← Cysteine/O‑acetylserine efflux protein
RA 3,912,321 T→G 20.4% S820A (TCG→GCG)  mexB_2 → Multidrug resistance protein MexB
RA 3,919,311 G→A 6.4% S275S (AGC→AGT CJ019_03703 ← hypothetical protein
RA 3,957,351 G→A 100% H100Y (CAC→TAC)  dmlR_13 ← HTH‑type transcriptional regulator DmlR
RA 3,969,776 A→C 35.9% L246R (CTG→CGG)  CJ019_03750 ← putative protein
RA 4,000,955 A→T 6.9% L189Q (CTA→CAA)  ggt_1 ← Glutathione hydrolase proenzyme
RA 4,029,463 A→C 26.5% D290A (GAC→GCC)  mcpU_3 → Methyl‑accepting chemotaxis protein McpU
RA 4,030,329 T→G 22.9% F579V (TTT→GTT)  mcpU_3 → Methyl‑accepting chemotaxis protein McpU
RA 4,079,259 G→A 5.9% A105T (GCC→ACC)  dadA → D‑amino acid dehydrogenase
RA 4,092,059 A→C 19.4% G92G (GGT→GGG CJ019_03853 ← 2‑haloacrylate reductase
RA 4,092,063 A→C 28.1% V91G (GTC→GGC)  CJ019_03853 ← 2‑haloacrylate reductase
RA 4,127,172 T→G 10.1% T148P (ACC→CCC)  nrtD_3 ← Nitrate import ATP‑binding protein NrtD
RA 4,130,129 T→G 18.3% A231A (GCT→GCG mtrA → HTH‑type transcriptional regulator MtrA
RA 4,131,763 T→G 27.6% E23A (GAG→GCG)  CJ019_03889 ← hypothetical protein
RA 4,135,607 A→C 29.7% Q32P (CAG→CCG)  tgnC → (Z)‑2‑((N‑methylformamido)methylene)‑5‑hydroxybu tyrolactone dehydrogenase
RA 4,163,919 C→T 7.7% intergenic (‑382/‑5) katG ← / → leuO_2 Catalase‑peroxidase/HTH‑type transcriptional regulator LeuO
RA 4,245,145 A→C 46.1% L178R (CTG→CGG)  CJ019_03982 ← Crotonyl‑CoA hydratase
RA 4,245,154 T→G 38.8% D175A (GAT→GCT)  CJ019_03982 ← Crotonyl‑CoA hydratase
RA 4,289,845 T→G 46.2% intergenic (+119/‑106) CJ019_04021 → / → gloA hypothetical protein/Lactoylglutathione lyase
RA 4,348,580 G→A 5.6% G45G (GGG→GGA cysT_1 → Sulfate transport system permease protein CysT
RA 4,662,146 T→C 7.4% I124T (ATC→ACC)  nuoM → NADH‑quinone oxidoreductase subunit M
RA 4,851,109 T→A 9.1% V35E (GTG→GAG)  CJ019_04549 → hypothetical protein
RA 4,874,567 A→C 34.1% intergenic (+13/‑174) CJ019_04568 → / → uacT_1 hypothetical protein/Uric acid transporter UacT
RA 4,884,468 T→A 19.0% intergenic (+45/‑194) glxR → / → ttuD 2‑hydroxy‑3‑oxopropionate reductase/Putative hydroxypyruvate reductase
RA 4,971,386 Δ1 bp 7.7% coding (329/1110 nt) flgI ← Flagellar P‑ring protein
RA 4,971,387 Δ1 bp 7.7% coding (328/1110 nt) flgI ← Flagellar P‑ring protein
RA 5,426,158 Δ1 bp 5.8% coding (186/519 nt) CJ019_05104 → hypothetical protein
RA 5,441,450 G→C 5.7% E377Q (GAG→CAG)  hemL → Glutamate‑1‑semialdehyde 2,1‑aminomutase
RA 5,585,616 T→A 6.2% D28V (GAC→GTC)  nudF ← ADP‑ribose pyrophosphatase
RA 5,685,825 G→C 36.5% P122P (CCG→CCC CJ019_05336 → hypothetical protein
RA 5,689,824 T→G 59.6% R238R (CGT→CGG pyrC' → Dihydroorotase‑like protein
RA 5,757,234 Δ1 bp 100% coding (776/834 nt) CJ019_05400 → hypothetical protein
RA 5,757,828 G→A 13.3% intergenic (+52/‑27) envC → / → CJ019_05402 Murein hydrolase activator EnvC/hypothetical protein
RA 5,830,444 C→T 7.3% P235L (CCC→CTC)  CJ019_05460 → putative zinc protease
RA 5,846,725 Δ1 bp 5.5% coding (847/1056 nt) CJ019_05475 ← hypothetical protein
RA 5,850,615 T→G 28.0% V236G (GTG→GGG)  CJ019_05478 → putative membrane protein
RA 5,922,380 C→T 7.3% L168L (CTG→CTA CJ019_05543 ← putative binding protein component of ABC iron transporter
RA 6,022,319 A→G 6.4% L326P (CTG→CCG)  puuC_2 ← NADP/NAD‑dependent aldehyde dehydrogenase PuuC
RA 6,133,865 C→G 10.5% P412R (CCT→CGT)  cusB → Cation efflux system protein CusB

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ KT2440 213568 213676 109 15 [11] [13] 14 [CJ019_00185] [CJ019_00185]
* * ÷ KT2440 277598 277691 94 14 [13] [13] 14 [betT2]–[tauD_2] [betT2],[tauD_2]
* * ÷ KT2440 492236 492350 115 18 [12] [13] 14 uvrY/potA_1 Response regulator UvrY/Spermidine/putrescine import ATP‑binding proteinPotA
* * ÷ KT2440 517196 517290 95 14 [12] [13] 14 tyrS/birA Tyrosine‑‑tRNA ligase/Bifunctional ligase/repressor BirA
* * ÷ KT2440 674232 674380 149 14 [13] [9] 15 [nimR_1]–[mcpH_2] [nimR_1],[mcpH_2]
* * ÷ KT2440 711474 711554 81 14 [13] [13] 14 [thiO] [thiO]
* * ÷ KT2440 948166 948218 53 14 [12] [11] 14 cyoE/alaC Protoheme IX farnesyltransferase/Glutamate‑pyruvate aminotransferase AlaC
* * ÷ KT2440 1304770 1304854 85 14 [13] [13] 14 CJ019_01223/rapA putative signaling protein/RNA polymerase‑associated protein RapA
* * ÷ KT2440 1360610 1360989 380 14 [13] [12] 14 [CJ019_01275] [CJ019_01275]
* * ÷ KT2440 1396593 1396680 88 15 [13] [13] 14 [mcpU_1] [mcpU_1]
* * ÷ KT2440 1456049 1456100 52 14 [13] [13] 15 patA/algL Peptidoglycan O‑acetyltransferase/Alginate lyase
* * ÷ KT2440 1499941 1500061 121 14 [13] [13] 18 [sspA] [sspA]
* * ÷ KT2440 1585631 1585700 70 14 [13] [12] 14 CJ019_01493 hypothetical protein
* * ÷ KT2440 1608691 1608816 126 15 [12] [13] 14 [CJ019_01513]–[CJ019_01514] [CJ019_01513],[CJ019_01514]
* * ÷ KT2440 1697661 1697724 64 14 [13] [13] 14 [CJ019_01593] [CJ019_01593]
* * ÷ KT2440 1705087 1705208 122 16 [12] [13] 14 ppc/adk Phosphoenolpyruvate carboxylase/Adenylate kinase
* * ÷ KT2440 1833103 1833181 79 14 [13] [12] 14 [resA_1]–[CJ019_01751] [resA_1],[CJ019_01751]
* * ÷ KT2440 1914565 1914639 75 14 [10] [13] 15 [ppsC_1] [ppsC_1]
* * ÷ KT2440 1923414 1923538 125 14 [13] [13] 16 [apeB] [apeB]
* * ÷ KT2440 1940792 1940945 154 17 [13] [13] 14 [CJ019_01857] [CJ019_01857]
* * ÷ KT2440 2288822 2288934 113 16 [11] [12] 17 CJ019_02171/CJ019_02172 hypothetical protein/hypothetical protein
* * ÷ KT2440 2942842 2942871 30 22 [6] [13] 14 CJ019_02780/CJ019_02781 hypothetical protein/8‑oxoguanine deaminase
* * ÷ KT2440 2944350 2944449 100 32 [13] [13] 14 [CJ019_02782] [CJ019_02782]
* * ÷ KT2440 3046205 3046347 143 14 [13] [13] 14 [rcsC_5]–[degU_2] [rcsC_5],[degU_2]
* * ÷ KT2440 3145262 3145390 129 14 [13] [13] 14 livH_4 High‑affinity branched‑chain amino acidtransport system permease protein LivH
* * ÷ KT2440 3280234 3280343 110 14 [13] [10] 14 [CJ019_03085] [CJ019_03085]
* * ÷ KT2440 3459849 3459932 84 14 [13] [11] 15 CJ019_03284 hypothetical protein
* * ÷ KT2440 3769399 3769476 78 14 [12] [13] 15 CJ019_03581/CJ019_03582 hypothetical protein/hypothetical protein
* * ÷ KT2440 3944707 3944794 88 17 [12] [12] 14 CJ019_03729/CJ019_03730 hypothetical protein/hypothetical protein
* * ÷ KT2440 4163797 4163866 70 22 [13] [13] 15 katG/leuO_2 Catalase‑peroxidase/HTH‑type transcriptional regulator LeuO
* * ÷ KT2440 4332083 4332168 86 16 [9] [11] 14 [CJ019_04068] [CJ019_04068]
* * ÷ KT2440 4346796 4346849 54 14 [13] [12] 14 nmoA Nitronate monooxygenase
* * ÷ KT2440 4497401 4497480 80 14 [13] [12] 14 [dsrE] [dsrE]
* * ÷ KT2440 4523640 4523714 75 16 [12] [13] 14 CJ019_04289/topB Acetyltransferase/DNA topoisomerase 3
* * ÷ KT2440 4734467 4734517 51 14 [13] [11] 14 gltA/CJ019_04468 Citrate synthase/hypothetical protein
* * ÷ KT2440 4790226 4790323 98 17 [13] [13] 14 [qseC_3] [qseC_3]
* * ÷ KT2440 4801805 4802165 361 14 [13] [12] 14 CJ019_04509/dsbD_2 hypothetical protein/Thiol:disulfide interchange protein DsbD
* * ÷ KT2440 4846236 4846359 124 14 [11] [11] 14 recR/ybaB Recombination protein RecR/Nucleoid‑associated protein YbaB
* * ÷ KT2440 4944736 4944877 142 15 [13] [13] 15 [CJ019_04642] [CJ019_04642]
* * ÷ KT2440 5167031 5167090 60 16 [13] [7] 17 fadD_1/fadD_2 Long‑chain‑fatty‑acid‑‑CoA ligase/Long‑chain‑fatty‑acid‑‑CoA ligase
* * ÷ KT2440 5188657 5188779 123 14 [12] [12] 14 [CJ019_04882]–[pxpC_2] [CJ019_04882],[pxpC_2]
* * ÷ KT2440 5193435 5193541 107 15 [13] [13] 14 [gltR_4]–[CJ019_04888] [gltR_4],[CJ019_04888]
* * ÷ KT2440 5250610 5250717 108 15 [12] [10] 16 [CJ019_04947] [CJ019_04947]
* * ÷ KT2440 5500270 5500467 198 14 [13] [13] 15 [CJ019_05176] [CJ019_05176]
* * ÷ KT2440 5551605 5551800 196 14 [11] [11] 15 [purA] [purA]
* * ÷ KT2440 5891553 5891657 105 16 [13] [13] 15 CJ019_05517/czcA_3 hypothetical protein/Cobalt‑zinc‑cadmium resistance protein CzcA
* * ÷ KT2440 5896967 5897035 69 14 [12] [10] 15 [CJ019_05521] [CJ019_05521]
* * ÷ KT2440 5990989 5991070 82 14 [13] [11] 14 [CJ019_05610] [CJ019_05610]
* * ÷ KT2440 6074800 6074878 79 14 [12] [13] 14 [CJ019_05690] [CJ019_05690]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? KT2440 4918800 =0 (0.000)63 (1.360) 24/226 NT 100% coding (7/789 nt) soj_1 Sporulation initiation inhibitor protein Soj
?KT2440 = 5539492 NA (NA)intergenic (‑358/+71) CJ019_03622/rlmB IS110 family transposase ISPpu9/23S rRNA (guanosine‑2'‑O‑)‑methyltransferaseRlmB
* ? KT2440 = 49188010 (0.000)47 (1.000) 33/228 NT 100% coding (6/789 nt) soj_1 Sporulation initiation inhibitor protein Soj
?KT2440 5216408 = NA (NA)intergenic (‑50/+386) rhaS_5/CJ019_01212 HTH‑type transcriptional activator RhaS/IS110 family transposase ISPpu9