breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 44,293 Δ1 bp 100% intergenic (+124/‑367) oprD_1 → / → czcR_2 Porin D/Transcriptional activator protein CzcR
RA 96,480 T→G 41.2% intergenic (+121/‑72) cynT → / → CJ019_00104 Carbonic anhydrase 1/hypothetical protein
RA 120,975 Δ1 bp 100% intergenic (+272/+100) polA → / ← engB DNA polymerase I/putative GTP‑binding protein EngB
RA 169,760 A→C 5.7% intergenic (+724/+2963) PP_RS08750 → / ← fhuA_1 tRNA‑Val/Ferrichrome outer membrane transporter/phagereceptor
RA 169,792 G→A 5.5% intergenic (+756/+2931) PP_RS08750 → / ← fhuA_1 tRNA‑Val/Ferrichrome outer membrane transporter/phagereceptor
RA 179,484 T→C 100% C316R (TGC→CGC)  CJ019_00182 → hypothetical protein
RA 189,506 T→C 15.2% T1455T (ACT→ACC CJ019_00185 → hypothetical protein
RA 362,975 G→C 6.3% intergenic (+27/‑49) CJ019_00336 → / → CJ019_00337 hypothetical protein/hypothetical protein
RA 382,484 T→G 39.9% S14A (TCC→GCC)  soxD → Sarcosine oxidase subunit delta
RA 399,292 A→C 34.9% intergenic (‑186/+122) CJ019_00365 ← / ← aceF hypothetical protein/Dihydrolipoyllysine‑residue acetyltransferasecomponent of pyruvate dehydrogenase complex
RA 399,304 T→G 37.2% intergenic (‑198/+110) CJ019_00365 ← / ← aceF hypothetical protein/Dihydrolipoyllysine‑residue acetyltransferasecomponent of pyruvate dehydrogenase complex
RA 444,535 A→C 23.5% L130V (TTG→GTG)  hdfR_1 ← HTH‑type transcriptional regulator HdfR
RA 477,777 A→T 6.8% A109A (GCT→GCA glpE ← Thiosulfate sulfurtransferase GlpE
RA 482,474 A→C 33.6% V67G (GTG→GGG)  surA_1 ← Chaperone SurA
RA 484,863 C→T 8.2% R203H (CGT→CAT)  lptD ← LPS‑assembly protein LptD
RA 603,170 G→A 8.5% H214H (CAC→CAT btuB_1 ← Vitamin B12 transporter BtuB
RA 634,781 Δ1 bp 7.8% coding (121/1089 nt) ydjJ ← putative zinc‑type alcohol dehydrogenase‑likeprotein YdjJ
RA 655,074 C→A 9.3% D311Y (GAC→TAC)  dinF ← DNA damage‑inducible protein F
RA 699,520 Δ1 bp 6.2% coding (1947/2301 nt) yebT → Intermembrane transport protein YebT
RA 813,275 C→T 6.9% G235G (GGC→GGT ada → Bifunctional transcriptional activator/DNArepair enzyme Ada
RA 868,338 A→C 56.0% intergenic (+27/+19) loiP → / ← mneA Metalloprotease LoiP/Putative manganese exporter
RA 990,702 T→A 18.0% intergenic (‑54/+175) CJ019_00935 ← / ← bfrD hypothetical protein/putative TonB‑dependent receptor BfrD
RA 1,212,957 A→T 7.3% L17Q (CTG→CAG)  ydgC ← Inner membrane protein YdgC
RA 1,269,009 C→T 8.1% intergenic (‑146/‑61) CJ019_01190 ← / → CJ019_01191 hypothetical protein/hypothetical protein
RA 1,313,950 T→G 42.8% R612R (AGG→CGG)  CJ019_01235 ← hypothetical protein
RA 1,357,456 C→A 8.6% L366M (CTG→ATG)  dctA2_1 → C4‑dicarboxylate transport protein 2
RA 1,388,322 T→G 36.8% L336V (TTG→GTG)  ruvB_1 → Holliday junction ATP‑dependent DNA helicaseRuvB
RA 1,508,681 T→G 18.1% V187G (GTG→GGG)  ftsI → Peptidoglycan D,D‑transpeptidase FtsI
RA 1,525,921 C→T 6.1% T292T (ACC→ACT secA → Protein translocase subunit SecA
RA 1,528,308 A→C 38.4% P87P (CCA→CCC argJ → Arginine biosynthesis bifunctional protein ArgJ
RA 1,593,255 A→G 6.7% S328P (TCC→CCC)  CJ019_01499 ← Beta‑xylosidase
RA 1,628,470 A→G 8.0% E221E (GAA→GAG era → GTPase Era
RA 1,630,540 A→C 36.1% L419R (CTG→CGG)  czcS ← Sensor protein CzcS
RA 1,670,690 A→C 33.2% intergenic (+27/‑104) thrC_2 → / → CJ019_01569 Threonine synthase/hypothetical protein
RA 1,699,833 T→C 7.9% F281S (TTC→TCC)  CJ019_01595 → hypothetical protein
RA 1,744,031 G→C 36.8% A92P (GCC→CCC)  CJ019_01649 → hypothetical protein
RA 1,805,331 C→T 100% R48H (CGC→CAC)  dmlR_4 ← HTH‑type transcriptional regulator DmlR
RA 2,134,732 T→G 24.1% L471V (TTG→GTG)  comEC → ComE operon protein 3
RA 2,138,813 C→T 6.3% A37V (GCT→GTT)  CJ019_02027 → Putative low molecular weight protein‑tyrosine‑phosphatase
RA 2,145,263 A→G 7.0% E297G (GAA→GGA)  rluC → Ribosomal large subunit pseudouridine synthaseC
RA 2,341,932 C→T 9.7% A361V (GCG→GTG)  bepE_2 → Efflux pump membrane transporter BepE
RA 2,371,171 G→A 8.2% R251H (CGC→CAC)  zntB → Zinc transport protein ZntB
RA 2,488,613 C→G 6.6% R101P (CGC→CCC)  CJ019_02346 ← hypothetical protein
RA 2,503,742 T→G 31.0% Q349P (CAG→CCG)  copA_2 ← Copper resistance protein A
RA 2,529,790 A→T 5.9% V769E (GTG→GAG)  mrpA ← Na( )/H( ) antiporter subunit A
RA 2,569,872 A→C 27.8% L518R (CTG→CGG)  acoR_3 ← Acetoin catabolism regulatory protein
RA 2,571,359 G→A 5.5% H22H (CAC→CAT acoR_3 ← Acetoin catabolism regulatory protein
RA 2,607,430 G→T 5.8% Q1104H (CAG→CAT CJ019_02463 → hypothetical protein
RA 2,671,714 A→G 9.7% D333G (GAC→GGC)  sasA_7 → Adaptive‑response sensory‑kinase SasA
RA 2,721,707 C→T 31.8% S126S (TCC→TCT gcvA_7 → Glycine cleavage system transcriptionalactivator
RA 2,911,037 C→T 8.7% Q2334* (CAG→TAG)  CJ019_02759 → hypothetical protein
RA 3,039,492 Δ1 bp 5.7% coding (68/786 nt) pstB3 → Phosphate import ATP‑binding protein PstB 3
RA 3,061,793 G→A 100% S592N (AGC→AAC)  qedA_2 → Quinoprotein alcohol dehydrogenase (cytochromec)
RA 3,202,757 A→T 23.4% V308V (GTA→GTT hcf136_2 → Ycf48‑like protein
RA 3,237,345 A→C 35.1% intergenic (+76/+25) dinG_3 → / ← CJ019_03036 3'‑5' exonuclease DinG/hypothetical protein
RA 3,308,718 A→C 36.3% R166R (CGT→CGG CJ019_03110 ← hypothetical protein
RA 3,491,323 T→G 47.1% intergenic (‑685/+36) CJ019_03306 ← / ← CJ019_03307 hypothetical protein/hypothetical protein
RA 3,568,762 A→G 6.6% M262T (ATG→ACG)  uspE_2 ← Universal stress protein E
RA 3,599,230 A→G 6.3% L197L (TTG→CTG)  xerC_4 ← Tyrosine recombinase XerC
RA 3,712,756 T→C 100% intergenic (‑299/‑48) paaF_1 ← / → yrdA_3 2,3‑dehydroadipyl‑CoA hydratase/Protein YrdA
RA 3,722,166 G→A 5.8% V13I (GTA→ATA)  CJ019_03540 → hypothetical protein
RA 3,763,787 A→C 28.2% E44A (GAG→GCG)  CJ019_03576 → hypothetical protein
RA 3,763,865 A→C 18.6% K70T (AAG→ACG)  CJ019_03576 → hypothetical protein
RA 3,947,782 A→C 29.0% L129R (CTG→CGG)  CJ019_03732 ← hypothetical protein
RA 3,956,826 G→C 100% H275D (CAT→GAT)  dmlR_13 ← HTH‑type transcriptional regulator DmlR
RA 3,989,982 A→C 39.1% intergenic (+15/+30) CJ019_03768 → / ← CJ019_03769 hypothetical protein/hypothetical protein
RA 4,029,445 A→C 17.9% Q284P (CAG→CCG)  mcpU_3 → Methyl‑accepting chemotaxis protein McpU
RA 4,042,017 A→C 24.4% D308A (GAC→GCC)  quiA → Quinate/shikimate dehydrogenase (quinone)
RA 4,077,164 T→G 35.6% L173R (CTG→CGG)  artJ_2 → ABC transporter arginine‑binding protein 1
RA 4,112,358 T→G 21.7% V468G (GTC→GGC)  iorB_2 → Isoquinoline 1‑oxidoreductase subunit beta
RA 4,135,607 A→C 49.6% Q32P (CAG→CCG)  tgnC → (Z)‑2‑((N‑methylformamido)methylene)‑5‑hydroxybu tyrolactone dehydrogenase
RA 4,151,360 A→C 15.2% V55G (GTG→GGG)  cmpR_5 ← HTH‑type transcriptional activator CmpR
RA 4,187,960 C→T 5.2% intergenic (‑239/+249) CJ019_03937 ← / ← CJ019_03938 hypothetical protein/hypothetical protein
RA 4,213,235 G→A 8.9% intergenic (‑279/‑94) CJ019_03953 ← / → CJ019_03954 hypothetical protein/hypothetical protein
RA 4,245,134 A→C 29.0% L182V (TTG→GTG)  CJ019_03982 ← Crotonyl‑CoA hydratase
RA 4,245,145 A→C 62.5% L178R (CTG→CGG)  CJ019_03982 ← Crotonyl‑CoA hydratase
RA 4,245,154 T→G 40.1% D175A (GAT→GCT)  CJ019_03982 ← Crotonyl‑CoA hydratase
RA 4,291,840 T→G 30.4% intergenic (‑142/‑20) sdhL ← / → CJ019_04025 Shikimate dehydrogenase‑like protein/hypothetical protein
RA 4,343,972 T→G 14.7% N258T (AAC→ACC)  tsdA ← Thiosulfate dehydrogenase
RA 4,546,287 T→C 6.0% intergenic (‑34/+64) rutR_2 ← / ← CJ019_04309 HTH‑type transcriptional regulator RutR/hypothetical protein
RA 4,647,997 C→T 7.1% G276G (GGC→GGT aceA → Isocitrate lyase
RA 4,690,862 T→G 40.6% L281R (CTG→CGG)  yhaJ_2 → HTH‑type transcriptional regulator YhaJ
RA 4,744,948 T→A 5.8% I291F (ATC→TTC)  proP_5 ← Proline/betaine transporter
RA 4,749,309 C→G 24.4% V301V (GTC→GTG macB → Macrolide export ATP‑binding/permease proteinMacB
RA 4,760,878 G→C 9.2% Q628H (CAG→CAC fpvA_3 → Ferripyoverdine receptor
RA 4,789,443 A→C 29.0% V230G (GTG→GGG)  dat ← Diaminobutyrate‑‑2‑oxoglutarateaminotransferase
RA 4,817,421 A→C 27.9% L2531R (CTG→CGG)  lgrB_2 ← Linear gramicidin synthase subunit B
RA 4,893,888 A→C 20.9% intergenic (+26/‑472) lrpC → / → pucI_2 HTH‑type transcriptional regulator LrpC/putative allantoin permease
RA 4,927,692 A→C 50.8% W194G (TGG→GGG)  srp54 ← Signal recognition particle 54 kDa protein
RA 4,959,063 G→A 100% Q40* (CAG→TAG)  norR_4 ← Anaerobic nitric oxide reductase transcriptionregulator NorR
RA 5,096,964 G→C 36.8% intergenic (+70/‑120) CJ019_04792 → / → CJ019_04793 Putative pterin‑4‑alpha‑carbinolaminedehydratase/hypothetical protein
RA 5,122,692 A→C 29.2% L80R (CTG→CGG)  slyA_2 ← Transcriptional regulator SlyA
RA 5,255,631 A→G 8.6% intergenic (‑73/‑23) metE ← / → CJ019_04952 5‑methyltetrahydropteroyltriglutamate‑‑homocystei ne methyltransferase/hypothetical protein
RA 5,267,269 A→C 30.9% D197A (GAC→GCC)  rlmG → Ribosomal RNA large subunit methyltransferase G
RA 5,358,311 T→G 23.8% H50P (CAC→CCC)  folP ← Dihydropteroate synthase
RA 5,546,108 T→G 20.4% V504G (GTG→GGG)  CJ019_05222 → hypothetical protein
RA 5,689,790 T→G 49.0% V227G (GTG→GGG)  pyrC' → Dihydroorotase‑like protein
RA 5,689,794 T→G 48.7% R228R (CGT→CGG pyrC' → Dihydroorotase‑like protein
RA 5,689,816 A→C 69.8% S236R (AGC→CGC)  pyrC' → Dihydroorotase‑like protein
RA 5,757,234 Δ1 bp 100% coding (776/834 nt) CJ019_05400 → hypothetical protein
RA 5,809,389 C→A 45.6% intergenic (+38/+53) ftsN → / ← CJ019_05436 Cell division protein FtsN/hypothetical protein
RA 5,829,038 C→T 9.7% P494L (CCG→CTG)  CJ019_05458 → hypothetical protein
RA 5,844,716 A→C 22.9% E529A (GAG→GCG)  ilvD → Dihydroxy‑acid dehydratase
RA 5,947,570 T→G 15.6% L169R (CTG→CGG) 
Q423P (CAG→CCG) 
CJ019_05568 →
CJ019_05569 ←
hypothetical protein
hypothetical protein
RA 5,975,241 T→G 43.3% L684R (CTG→CGG)  sasA_19 → Adaptive‑response sensory‑kinase SasA
RA 5,975,263 A→C 34.0% P691P (CCA→CCC sasA_19 → Adaptive‑response sensory‑kinase SasA
RA 5,975,297 A→C 19.5% S703R (AGC→CGC)  sasA_19 → Adaptive‑response sensory‑kinase SasA
RA 6,005,873 C→T 7.4% A297V (GCG→GTG)  rep_2 → ATP‑dependent DNA helicase Rep
RA 6,021,514 T→G 33.9% S60R (AGC→CGC)  ycaD_4 ← putative MFS‑type transporter YcaD
RA 6,021,519 A→C 49.2% L58R (CTG→CGG)  ycaD_4 ← putative MFS‑type transporter YcaD
RA 6,048,049 T→A 6.5% G186G (GGT→GGA tolQ_3 → Tol‑Pal system protein TolQ
RA 6,073,133 A→T 5.9% intergenic (‑73/‑289) abaF_2 ← / → CJ019_05688 Fosfomycin resistance protein AbaF/putative acyl‑CoA thioester hydrolase
RA 6,139,542 T→G 16.9% N2T (AAC→ACC)  CJ019_05747 ← hypothetical protein

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ KT2440 87996 88060 65 15 [14] [14] 21 [CJ019_00094]–[CJ019_00095] [CJ019_00094],[CJ019_00095]
* * ÷ KT2440 161217 161368–161317 101–152 17 [14] [14] 16 [uctC_1] [uctC_1]
* * ÷ KT2440 277598 277669 72 15 [14] [14] 15 [betT2]–[tauD_2] [betT2],[tauD_2]
* * ÷ KT2440 346103 346210 108 16 [13] [14] 16 [btuD_1] [btuD_1]
* * ÷ KT2440 517190 517335 146 19 [10] [14] 16 tyrS/birA Tyrosine‑‑tRNA ligase/Bifunctional ligase/repressor BirA
* * ÷ KT2440 661101 661204 104 16 [14] [14] 15 yfhM/CJ019_00619 Alpha‑2‑macroglobulin/hypothetical protein
* * ÷ KT2440 674266 674390 125 15 [14] [14] 15 [nimR_1]–[mcpH_2] [nimR_1],[mcpH_2]
* * ÷ KT2440 711463 711556 94 16 [14] [14] 17 [thiO] [thiO]
* * ÷ KT2440 789825 789954 130 16 [14] [13] 15 [CJ019_00751] [CJ019_00751]
* * ÷ KT2440 860948 861076 129 22 [14] [10] 15 [CJ019_00824] [CJ019_00824]
* * ÷ KT2440 1351811 1351917 107 15 [13] [14] 15 entD Enterobactin synthase component D
* * ÷ KT2440 1396579 1396677 99 15 [14] [14] 15 [CJ019_01313]–[mcpU_1] [CJ019_01313],[mcpU_1]
* * ÷ KT2440 1457227 1457284 58 15 [9] [13] 15 [algX] [algX]
* * ÷ KT2440 1480084 1480240 157 18 [14] [14] 15 [degS] [degS]
* * ÷ KT2440 1492461 1492587 127 16 [11] [14] 15 [CJ019_01406] [CJ019_01406]
* * ÷ KT2440 1585616 1585700 85 15 [14] [14] 16 CJ019_01493 hypothetical protein
* * ÷ KT2440 1697661 1697728 68 17 [14] [14] 15 [CJ019_01593] [CJ019_01593]
* * ÷ KT2440 1833079 1833194 116 15 [14] [13] 16 [resA_1]–[CJ019_01751] [resA_1],[CJ019_01751]
* * ÷ KT2440 1835810 1835921 112 16 [14] [14] 16 barA Signal transduction histidine‑protein kinaseBarA
* * ÷ KT2440 1923404 1923513 110 16 [13] [14] 16 [apeB] [apeB]
* * ÷ KT2440 2234310 2234454 145 15 [14] [14] 15 [gltX] [gltX]
* * ÷ KT2440 2288824 2288922 99 15 [14] [6] 15 CJ019_02171/CJ019_02172 hypothetical protein/hypothetical protein
* * ÷ KT2440 2660996 2661173 178 15 [14] [14] 15 [prpF] [prpF]
* * ÷ KT2440 2830107 2830287 181 15 [13] [12] 15 gabD1/namA_3 Succinate‑semialdehyde dehydrogenase [NADP( )]1/NADPH dehydrogenase
* * ÷ KT2440 3046190 3046338 149 15 [10] [14] 15 [rcsC_5]–[degU_2] [rcsC_5],[degU_2]
* * ÷ KT2440 3459859 3459932 74 15 [14] [12] 15 CJ019_03284 hypothetical protein
* * ÷ KT2440 3769400 3769474 75 17 [12] [13] 15 CJ019_03581/CJ019_03582 hypothetical protein/hypothetical protein
* * ÷ KT2440 3839285 3839419 135 15 [13] [12] 15 [argP_2] [argP_2]
* * ÷ KT2440 3944694 3944814 121 16 [13] [14] 15 CJ019_03729/CJ019_03730 hypothetical protein/hypothetical protein
* * ÷ KT2440 4163799 4163871 73 16 [14] [14] 16 katG/leuO_2 Catalase‑peroxidase/HTH‑type transcriptional regulator LeuO
* * ÷ KT2440 4332104 4332161 58 15 [14] [12] 15 aes/CJ019_04068 Acetyl esterase/hypothetical protein
* * ÷ KT2440 4333686 4333773 88 18 [13] [14] 16 ribBA_2/potD_2 Riboflavin biosynthesis protein RibBA/Spermidine/putrescine‑binding periplasmicprotein
* * ÷ KT2440 4523639 4523707 69 15 [14] [13] 17 [CJ019_04289] [CJ019_04289]
* * ÷ KT2440 4734445 4734513 69 16 [14] [13] 15 gltA/CJ019_04468 Citrate synthase/hypothetical protein
* * ÷ KT2440 4790231 4790302 72 15 [14] [14] 15 dat/qseC_3 Diaminobutyrate‑‑2‑oxoglutarateaminotransferase/Sensor protein QseC
* * ÷ KT2440 4801765 4802143 379 15 [12] [14] 15 CJ019_04509/dsbD_2 hypothetical protein/Thiol:disulfide interchange protein DsbD
* * ÷ KT2440 4944744 4944839 96 15 [14] [13] 17 [CJ019_04642] [CJ019_04642]
* * ÷ KT2440 5193421 5193546 126 15 [14] [14] 15 [gltR_4]–[CJ019_04888] [gltR_4],[CJ019_04888]
* * ÷ KT2440 5453344 5453464 121 15 [12] [14] 25 [lptE]–[holA] [lptE],[holA]
* * ÷ KT2440 5500276 5500472 197 15 [14] [14] 15 [CJ019_05176] [CJ019_05176]
* * ÷ KT2440 5646783 5646868 86 19 [14] [14] 16 CJ019_05302/CJ019_05303 hypothetical protein/hypothetical protein
* * ÷ KT2440 5891553 5891655 103 16 [12] [13] 15 CJ019_05517/czcA_3 hypothetical protein/Cobalt‑zinc‑cadmium resistance protein CzcA
* * ÷ KT2440 5896971 5897040 70 15 [13] [13] 19 [CJ019_05521] [CJ019_05521]
* * ÷ KT2440 5914023 5914094 72 15 [14] [12] 15 CJ019_05537 hypothetical protein

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? KT2440 4918800 =0 (0.000)59 (1.150) 27/228 NT 100% coding (7/789 nt) soj_1 Sporulation initiation inhibitor protein Soj
?KT2440 = 5539492 NA (NA)intergenic (‑358/+71) CJ019_03622/rlmB IS110 family transposase ISPpu9/23S rRNA (guanosine‑2'‑O‑)‑methyltransferaseRlmB
* ? KT2440 = 49188010 (0.000)47 (0.910) 28/230 NT 100% coding (6/789 nt) soj_1 Sporulation initiation inhibitor protein Soj
?KT2440 5216408 = NA (NA)intergenic (‑50/+386) rhaS_5/CJ019_01212 HTH‑type transcriptional activator RhaS/IS110 family transposase ISPpu9